Study on capillary electrophoresis-amperometric detection profiles of different parts of Morus alba L.

被引：47

作者：

Chu, Qingcui ^{[1
]}

Lin, Miao ^{[1
]}

Tian, Xiuhui ^{[1
]}

Ye, Jiannong ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1116卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; amperometric detection; CE-AD profiles; Morus alba L; mulberry;

D O I：

10.1016/j.chroma.2006.03.118

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been developed for the determination of the pharmacologically active ingredients in different parts of Morus alba L. after a relatively simple extraction procedure. This method was also used in the comparison of bioactive constituent difference in the five parts, based on their electropherograms or characteristic "CE-AD profiles". The effects of several factors such as the acidity and concentration of running buffer, separation voltage, applied potential and injection time were investigated to find the optimum conditions. Method detection limits (S/N = 3) ranged from 1.5 x 10(-7) to 1.4 x 10(-8) g/mL for all 10 analytes, and the assay results were satisfactory. (c) 2006 Published by Elsevier B.V.

引用

页码：286 / 290

页数：5

共 29 条

[1]

CAO HY, 1999, MED MAT MED RES, V10, P626

[2]

Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison [J].