Synthesis and characterization of ferrocene-labeled oligodeoxynucleotides

被引：44

作者：

Beilstein, AE ^{[1
]}

Grinstaff, MW ^{[1
]}

机构：

[1] Duke Univ, Dept Chem, Paul M Gross Chem Lab, Durham, NC 27708 USA

来源：

JOURNAL OF ORGANOMETALLIC CHEMISTRY | 2001年 / 637卷

关键词：

ferrocene; oligodeoxynucleotides; on-column; solid-phase; cross-coupling; synthesis; bioorganometallic chemistry;

D O I：

10.1016/S0022-328X(01)00943-3

中图分类号：

O61 [无机化学];

学科分类号：

070301 [无机化学]; 081704 [应用化学];

摘要：

Using a facile on-column derivatization procedure, oligodeoxynucleotides (ODNs) are labeled with a ferrocene derivative at specific sites. A Sonagashira cross-coupling reaction, using Pd(PPh3)(4) and CuI, links ferrocene propargylamide to a halogenated base, such as 2'-deoxy-5-iodouridine or 8-bromo-2'-deoxyadenosine, which has been previously incorporated in the ODN strand. The structure and stability of the DNA duplex are not significantly altered after site-specific labeling with ferrocene, as demonstrated by T-m and CD spectroscopy. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：398 / 406

页数：9

共 53 条

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A SIMPLE SPECIFIC LABELING FOR OLIGONUCLEOTIDES BY BATHOPHENANTHROLINE-RU(II) COMPLEXES AS NONRADIOACTIVE LABEL MOLECULES [J].