KCO1 is a component of the slow-vacuolar (SV) ion channel

被引：47

作者：

Schönknecht, G

Spoormaker, P

Steinmeyer, R

Brüggeman, L

Ache, P

Dutta, R

Reintanz, B

Godde, M

Hedrich, R

Palme, K

机构：

[1] Univ Wurzburg, Lehrstuhl Mol Pflanzenphysiol & Biophys, Julius von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany

[2] Oklahoma State Univ, Dept Bot, Stillwater, OK 74078 USA

[3] Max Planck Gesell, Max Delbruck Lab, D-50829 Cologne, Germany

来源：

FEBS LETTERS | 2002年 / 511卷 / 1-3期

关键词：

two-pore domain K+ channel; vacuolar membrane; patch-clamp; green fluorescence protein; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(01)03273-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：28 / 32

页数：5

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