A Molecular Link between miRISCs and Deadenylases Provides New Insight into the Mechanism of Gene Silencing by MicroRNAs

被引：43

作者：

Braun, Joerg E. ^{[1
]}

Huntzinger, Eric ^{[1
]}

Izaurralde, Elisa ^{[1
]}

机构：

[1] Max Planck Inst Dev Biol, Dept Biochem, D-72076 Tubingen, Germany

来源：

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY | 2012年 / 4卷 / 12期

关键词：

MEDIATED TRANSLATIONAL REPRESSION; MESSENGER-RNA DEADENYLATION; C-TERMINAL DOMAIN; CAENORHABDITIS-ELEGANS; LET-7; MICRORNA; REQUIRES GW182; P-BODIES; ARGONAUTE; PROTEIN; TARGET;

D O I：

10.1101/cshperspect.a012328

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

MicroRNAs (miRNAs) are a large family of endogenous noncoding RNAs that, together with the Argonaute family of proteins (AGOs), silence the expression of complementary mRNA targets posttranscriptionally. Perfectly complementary targets are cleaved within the base-paired region by catalytically active AGOs. In the case of partially complementary targets, however, AGOs are insufficient for silencing and need to recruit a protein of the GW182 family. GW182 proteins induce translational repression, mRNA deadenylation and exonucleolytic target degradation. Recent work has revealed a direct molecular link between GW182 proteins and cellular deadenylase complexes. These findings shed light on how miRNAs bring about target mRNA degradation and promise to further our understanding of the mechanism of miRNA-mediated repression.

引用

页数：15

共 87 条

[1]

Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes [J].

Aukerman, MJ ;

Sakai, H .

PLANT CELL, 2003, 15 (11) :2730-2741

[2]

Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation [J].

Bagga, S ;

Bracht, J ;

Hunter, S ;

Massirer, K ;

Holtz, J ;

Eachus, R ;

Pasquinelli, AE .

CELL, 2005, 122 (04) :553-563

[3]

MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[4]

Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish [J].

Bazzini, Ariel A. ;

Lee, Miler T. ;

Giraldez, Antonio J. .

SCIENCE, 2012, 336 (6078) :233-237

[5]

MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes [J].

Behm-Ansmant, Isabelle ;

Rehwinkel, Jan ;

Doerks, Tobias ;

Stark, Alexander ;

Bork, Peer ;

Izaurralde, Elisa .

GENES & DEVELOPMENT, 2006, 20 (14) :1885-1898

[6]

GW182 Proteins Directly Recruit Cytoplasmic Deadenylase Complexes to miRNA Targets [J].

Braun, Joerg E. ;

Huntzinger, Eric ;

Fauser, Maria ;

Izaurralde, Elisa .

MOLECULAR CELL, 2011, 44 (01) :120-133

[7]

Widespread translational inhibition by plant miRNAs and siRNAs [J].

Brodersen, Peter ;

Sakvarelidze-Achard, Lali ;

Bruun-Rasmussen, Marianne ;

Dunoyer, Patrice ;

Yamamoto, Yoshiharu Y. ;

Sieburth, Leslie ;

Voinnet, Olivier .

SCIENCE, 2008, 320 (5880) :1185-1190

[8]

miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs [J].

Chekulaeva, Marina ;

Mathys, Hansruedi ;

Zipprich, Jakob T. ;

Attig, Jan ;

Colic, Marija ;

Parker, Roy ;

Filipowicz, Witold .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2011, 18 (11) :1218-U62

[9]

Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila [J].

Chekulaeva, Marina ;

Filipowicz, Witold ;

Parker, Roy .

RNA, 2009, 15 (05) :794-803

[10]

Mechanisms of deadenylation-dependent decay [J].

Chen, Chyi-Ying A. ;

Shyu, Ann-Bin .

WILEY INTERDISCIPLINARY REVIEWS-RNA, 2011, 2 (02) :167-183

← 1 2 3 4 5 6 7 8 9 →