Widespread translational inhibition by plant miRNAs and siRNAs

被引：1321

作者：

Brodersen, Peter ^{[1
]}

Sakvarelidze-Achard, Lali ^{[1
]}

Bruun-Rasmussen, Marianne ^{[1
]}

Dunoyer, Patrice ^{[1
]}

Yamamoto, Yoshiharu Y. ^{[2
]}

Sieburth, Leslie ^{[3
]}

Voinnet, Olivier ^{[1
]}

机构：

[1] CNRS, Inst Biol Mol Plantes, Unite Propre Rech 2357, F-67084 Strasbourg, France

[2] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Aichi 4648602, Japan

[3] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA

来源：

SCIENCE | 2008年 / 320卷 / 5880期

关键词：

D O I：

10.1126/science.1159151

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

High complementarity between plant microRNAs ( miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA- guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA ( siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule- severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE ( VCS)/ Ge- 1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.

引用

页码：1185 / 1190

页数：6

共 44 条

[1]

Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes [J].

Aukerman, MJ ;

Sakai, H .

PLANT CELL, 2003, 15 (11) :2730-2741

[2]

MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3]

Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits rnicroRNAs and short interfering RNAs [J].

Baumberger, N ;

Baulcombe, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (33) :11928-11933

[4]

Relief of microRNA-mediated translational repression in human cells subjected to stress [J].

Bhattacharyya, Suvendra N. ;

Habermacher, Regula ;

Martine, Ursula ;

Closs, Ellen I. ;

Filipowicz, Witold .

CELL, 2006, 125 (06) :1111-1124

[5]

The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth [J].

Bouquin, T ;

Mattsson, O ;

Næsted, H ;

Foster, R ;

Mundy, J .

JOURNAL OF CELL SCIENCE, 2003, 116 (05) :791-801

[6]

Specialization and evolution of endogenous small RNA pathways [J].

Chapman, Elisabeth J. ;

Carrington, James C. .

NATURE REVIEWS GENETICS, 2007, 8 (11) :884-896

[7]

The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification [J].

Cook, HA ;

Koppetsch, BS ;

Wu, J ;

Theurkauf, WE .

CELL, 2004, 116 (06) :817-829

[8]

Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases [J].

Dugas, Diana V. ;

Bartel, Bonnie .

PLANT MOLECULAR BIOLOGY, 2008, 67 (04) :403-417

[9]

Intra- and intercellular RNA interference in Arabidopsis thaliana requires components of the microRNA and heterochromatic silencing pathways [J].

Dunoyer, Patrice ;

Himber, Christophe ;

Ruiz-Ferrer, Virginia ;

Alioua, Abdelmalek ;

Voinnet, Olivier .

NATURE GENETICS, 2007, 39 (07) :848-856

[10]

Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing [J].

Eulalio, Ana ;

Rehwinkel, Jan ;

Stricker, Mona ;

Huntzinger, Eric ;

Yang, Schu-Fee ;

Doerks, Tobias ;

Dorner, Silke ;

Bork, Peer ;

Boutros, Michael ;

Izaurralde, Elisa .

GENES & DEVELOPMENT, 2007, 21 (20) :2558-2570

← 1 2 3 4 5 →