Purification, cloning, and synthesis of a novel salivary anti-thrombin from the mosquito Anopheles albimanus

被引：79

作者：

Valenzuela, JG ^{[1
]}

Francischetti, IMB ^{[1
]}

Ribeiro, JMC ^{[1
]}

机构：

[1] NIAID, Parasit Dis Lab, Sect Med Entomol, Bethesda, MD 20892 USA

来源：

BIOCHEMISTRY | 1999年 / 38卷 / 34期

关键词：

D O I：

10.1021/bi990761i

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An anti-thrombin peptide (anophelin) was isolated from the salivary glands of the mosquito Anopheles albimanus through molecular sieving and reverse-phase high-performance liquid chromatography. The purified peptide inhibited thrombin-induced platelet aggregation, thrombin esterolytic activity on a synthetic substrate, and thrombin cleavage of fibrinogen. The purified anti-thrombin had a molecular mass of 6342.4 Da. Its amino terminus was blocked, but internal sequence yielded three peptide sequences, which were used to design oligonucleotide probes for polymerase chain reaction amplification of salivary gland cDNA and isolation of the full-length clone. Analysis of the sequence of anophelin shows no similarities to any other anti-thrombin peptides. Anophelin was successfully synthesized and characterized to be a tight-binding, specific, and novel inhibitor of thrombin.

引用

页码：11209 / 11215

页数：7

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