Mining of a streptothricin gene cluster from Streptomyces sp TP-A0356 genome via heterologous expression

被引：70

作者：

Li JinE ^{[1
]}

Guo ZhengYan ^{[1
]}

Huang Wei ^{[2
]}

Meng XiangXi ^{[1
,3
]}

Ai GuoMin ^{[1
]}

Tang GongLi ^{[2
]}

Chen YiHua ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2013年 / 56卷 / 07期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

genome mining; streptothricin; Streptomyces; heterologous expression; biosynthesis; BETA-LYSINE; NIKKOMYCIN BIOSYNTHESIS; RESISTANCE; SEQUENCE; PATHWAY; REGULATOR;

D O I：

10.1007/s11427-013-4504-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying beta-lysines for the poly-beta-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence.

引用

页码：619 / 627

页数：9

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