Fabrication of high quality microarrays

被引：142

作者：

Dufva, M ^{[1
]}

机构：

[1] Tech Univ Denmark, Microarray Technol Grp, Dept Micro & Nanotechnol, DK-2800 Kongens Lyngby, Denmark

来源：

BIOMOLECULAR ENGINEERING | 2005年 / 22卷 / 5-6期

关键词：

DNA microarrays; fabrication; immobilisation chemistry; optimisation;

D O I：

10.1016/j.bioeng.2005.09.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fabrication of DNA microarray demands that between ten (diagnostic microarrays) and many hundred thousands of probes (research or screening microarrays) are efficiently immobilised to a glass or plastic surface using a suitable chemistry. DNA microarray performance is measured by parameters like array geometry, spot density, spot characteristics (morphology, probe density and hybridised density), background, specificity and sensitivity. At least 13 factors affect these parameters and factors affecting fabrication of microarrays are used in this review to compare different fabrication methods (spotted microarrays and in situ synthesis of microarrays) and immobilisation chemistries. (C) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：173 / 184

页数：12

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