Enzyme immunoassay by capillary electrophoresis with laser induced fluorescence detection

被引：12

作者：

Koizumi, A ^{[1
]}

Morita, T ^{[1
]}

Murakami, Y ^{[1
]}

Morita, Y ^{[1
]}

Sakaguchi, T ^{[1
]}

Yokoyama, K ^{[1
]}

Tamiya, E ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Tatsunokuchi, Ishikawa 9231292, Japan

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 399卷 / 1-2期

关键词：

alkaline phosphatase; isoenzyme; B/F separation clinical test;

D O I：

10.1016/S0003-2670(99)00576-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antibody (Ab) labeled with alkaline phosphatase was separated and detected by a capillary electrophoresis system equipped with a laser induced fluorescence detection system. The combination of B/F separation and the LIF detection of the label enzyme is potentially useful for a highly sensitive detection of antigen (Ag). After B/F separation by capillary electrophoresis (CE), label enzymes catalyze the hydrolysis of fluorescein diphosphate in the running buffer for several minutes, producing fluorescein. Experiments with various ratios of Ab to Ag revealed that the faster peak was the complex of Ab and Ag, and the other was free Ab. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：63 / 68

页数：6

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