Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes

被引:305
作者
Gur, Y [1 ]
Breitbart, H [1 ]
机构
[1] Bar Ilan Univ, Fac Life Sci, IL-52900 Ramat Gan, Israel
关键词
sperm; protein translation; capacitation; fertilization; mitochondrial ribosomes;
D O I
10.1101/gad.367606
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It is widely accepted that spermatozoa are translationally silent. The present study demonstrates, for the first time, incorporation of labeled amino acids into polypeptides during sperm capacitation, which was completely inhibited by mitochondrial translation inhibitors but not by the cytoplasmic translation inhibitor. Unlike 80S cytoplasmic ribosomes, 55S mitochondrial ribosomes were present in polysomal fractions, indicating that these ribosomes are actively involved in protein translation in spermatozoa. Inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rate. Thus, contrary to the accepted dogma, nuclear genes are expressed as proteins in sperm during their residence in the female reproductive tract until fertilization.
引用
收藏
页码:411 / 416
页数:6
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