Two modes of sulfite oxidation in the extremely thermophilic and acidophilic archaeon Acidianus ambivalens

被引:34
作者
Zimmermann, P [1 ]
Laska, S [1 ]
Kletzin, A [1 ]
机构
[1] Tech Univ Darmstadt, Inst Mikrobiol & Genet, D-64287 Darmstadt, Germany
关键词
archaea; sulfite : acceptor oxidoreductase; adenylylsulfate; phosphate : adenyltransferase; ADP sulfurylase; adenylylsulfate reductase; 5; 5(1); P-4-di(adenosine-5 ') tetraphosphate synthase; 5-P-1; P-4-di(adenosine-5; tetraphosphate; pyrophosphohydrolase; adenylate kinase;
D O I
10.1007/s002030050743
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sulfite-oxidizing enzyme activities were analyzed in cell-free extracts of aerobically frown cells of Acidianus ambivalens, an extremely thermophilic and chemolithoautotrophic archaeon. In the membrane and cytoplasmic fractions, two distinct enzyme activities were found. In the membrane fraction, a sulfite:acceptor oxidoreductase activity was found [530 mU (mg protein)(-1); apparent K-m for sulfite, 3.6 mM]. In the cytoplasmic fraction the following enzyme activities were found and are indicative of an oxidative adenylylsulfate pathway: adenylylsulfate reductase [138 mU (mg protein)(-1)], adenylylsulfate:phosphate adenyltransferase ["ADP sulfurylase"; 86 mU (mg protein)(-1)], adenylate kinase [650 mU (mg protein)(-1)], and rhodanese [thiosulfate sulfur transferase, 9.2 mU (mg protein)(-1)]. In addition, 5',5"'-P-1,P-4-di(adenosine-5') tetraphosphate (Ap(4)A) synthase and Ap(4)A pyrophosphohydrolase activities were detected.
引用
收藏
页码:76 / 82
页数:7
相关论文
共 34 条
[1]  
ANEMULLER S, 1994, FEMS MICROBIOL LETT, V117, P275, DOI 10.1111/j.1574-6968.1994.tb06779.x
[2]   Adenylate kinase from Sulfolobus acidocaldarius: Expression in Escherichia coli and characterization by Fourier transform infrared spectroscopy [J].
Bonisch, H ;
Backmann, J ;
Kath, T ;
Naumann, D ;
Schafer, G .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 333 (01) :75-84
[3]   SULFUR OXIDATION BY PHOTOTROPHIC BACTERIA [J].
BRUNE, DC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 975 (02) :189-221
[4]  
BRUSER T, 1999, BIOSPEKTRUM SONDERAU, P60
[5]  
COHEN HJ, 1971, J BIOL CHEM, V246, P359
[6]  
DAHL C, 1989, Z NATURFORSCH C, V44, P617
[7]  
DAHL C, 1994, METHOD ENZYMOL, V243, P400
[8]  
DANE E, 1985, KLEINES CHEM PRAKTIK
[9]  
Friedrich CG, 1998, ADV MICROB PHYSIOL, V39, P235
[10]   16S rDNA-based phylogeny of the archaeal order Sulfolobales and reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb nov [J].
Fuchs, T ;
Huber, H ;
Burggraf, S ;
Stetter, KO .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (01) :56-60