Two modes of sulfite oxidation in the extremely thermophilic and acidophilic archaeon Acidianus ambivalens

被引：34

作者：

Zimmermann, P ^{[1
]}

Laska, S ^{[1
]}

Kletzin, A ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Inst Mikrobiol & Genet, D-64287 Darmstadt, Germany

来源：

ARCHIVES OF MICROBIOLOGY | 1999年 / 172卷 / 02期

关键词：

archaea; sulfite : acceptor oxidoreductase; adenylylsulfate; phosphate : adenyltransferase; ADP sulfurylase; adenylylsulfate reductase; 5; 5(1); P-4-di(adenosine-5 ') tetraphosphate synthase; 5-P-1; P-4-di(adenosine-5; tetraphosphate; pyrophosphohydrolase; adenylate kinase;

D O I：

10.1007/s002030050743

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sulfite-oxidizing enzyme activities were analyzed in cell-free extracts of aerobically frown cells of Acidianus ambivalens, an extremely thermophilic and chemolithoautotrophic archaeon. In the membrane and cytoplasmic fractions, two distinct enzyme activities were found. In the membrane fraction, a sulfite:acceptor oxidoreductase activity was found [530 mU (mg protein)(-1); apparent K-m for sulfite, 3.6 mM]. In the cytoplasmic fraction the following enzyme activities were found and are indicative of an oxidative adenylylsulfate pathway: adenylylsulfate reductase [138 mU (mg protein)(-1)], adenylylsulfate:phosphate adenyltransferase ["ADP sulfurylase"; 86 mU (mg protein)(-1)], adenylate kinase [650 mU (mg protein)(-1)], and rhodanese [thiosulfate sulfur transferase, 9.2 mU (mg protein)(-1)]. In addition, 5',5"'-P-1,P-4-di(adenosine-5') tetraphosphate (Ap(4)A) synthase and Ap(4)A pyrophosphohydrolase activities were detected.

引用

页码：76 / 82

页数：7

共 34 条

[11]

GURANOWSKI A, 1983, J BIOL CHEM, V258, P4784

[12] IDENTIFICATION, CLONING, AND EXPRESSION OF THE GENE FOR ADENYLATE KINASE FROM THE THERMOACIDOPHILIC ARCHAEBACTERIUM SULFOLOBUS-ACIDOCALDARIUS [J].

KATH, T ;

SCHMID, R ;

SCHAFER, G .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 307 (02) :405-410

[13]

KELLY DP, 1994, METHOD ENZYMOL, V243, P501

[14] Thermodynamic aspects of energy conservation by chemolithotrophic sulfur bacteria in relation to the sulfur oxidation pathways [J].

Kelly, DP .

ARCHIVES OF MICROBIOLOGY, 1999, 171 (04) :219-229

[15] Molecular basis of sulfite oxidase deficiency from the structure of sulfite oxidase [J].

Kisker, C ;

Schindelin, H ;

Pacheco, A ;

Wehbi, WA ;

Garrett, RM ;

Rajagopalan, KV ;

Enemark, JH ;

Rees, DC .

CELL, 1997, 91 (07) :973-983

[16] MOLECULAR CHARACTERIZATION OF THE SOR GENE, WHICH ENCODES THE SULFUR OXYGENASE REDUCTASE OF THE THERMOACIDOPHILIC ARCHAEUM DESULFUROLOBUS-AMBIVALENS [J].

KLETZIN, A .

JOURNAL OF BACTERIOLOGY, 1992, 174 (18) :5854-5859

[17]

KLETZIN A, 1994, SYST APPL MICROBIOL, V16, P534

[18] COUPLED ENZYMATIC PRODUCTION OF SULFITE, THIOSULFATE, AND HYDROGEN-SULFIDE FROM SULFUR - PURIFICATION AND PROPERTIES OF A SULFUR OXYGENASE REDUCTASE FROM THE FACULTATIVELY ANAEROBIC ARCHAEBACTERIUM DESULFUROLOBUS-AMBIVALENS [J].

KLETZIN, A .

JOURNAL OF BACTERIOLOGY, 1989, 171 (03) :1638-1643

[19] ARCHAEBACTERIAL ADENYLATE KINASE FROM THE THERMOACIDOPHILE SULFOLOBUS-ACIDOCALDARIUS - PURIFICATION, CHARACTERIZATION, AND PARTIAL SEQUENCE [J].

LACHER, K ;

SCHAFER, G .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 302 (02) :391-397

[20]

LAMPREIA J, 1994, METHOD ENZYMOL, V243, P241

← 1 2 3 4 →