Assessment of immunity to mycobacterial infection with luciferase reporter constructs

被引：161

作者：

Snewin, VA ^{[1
]}

Gares, MP ^{[1
]}

Gaora, PO ^{[1
]}

Hasan, Z ^{[1
]}

Brown, IN ^{[1
]}

Young, DB ^{[1
]}

机构：

[1] Imperial Coll Sch Med, Dept Infect Dis & Microbiol, London W2 1PG, England

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/IAI.67.9.4586-4593.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines, With the aim of developing assays that might provide a surrogate measure of protective immunity, rye have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacterial immunity in model systems. Measurement of luminescence was shown to provide a rapid and simple alternative to the counting of CFU as a means of monitoring mycobacterial viability. We describe optimization of a luciferase reporter strain of Mycobacterium tuberculosis and demonstrate its application for the study of mycobacterial interactions with host cells in tissue culture and the rapid assessment of vaccine efficacy in a murine model.

引用

页码：4586 / 4593

页数：8

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