Evidence for production of paralytic shellfish toxins by bacteria associated with Alexandrium spp (Dinophyta) in culture

被引:122
作者
Gallacher, S
Flynn, KJ
Franco, JM
Brueggemann, EE
Hines, HB
机构
[1] UNIV COLL SWANSEA, SCH BIOL SCI, SWANSEA ALGAL RES UNIT, SWANSEA SA2 8PP, W GLAM, WALES
[2] CSIC, INST INVEST MARINAS, VIGO 36208, SPAIN
[3] USA, MED RES INST INFECT DIS, DIV TOXINOL, FREDERICK, MD 21702 USA
关键词
D O I
10.1128/AEM.63.1.239-245.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A substantial proportion of bacteria from five Alexandrium cultures originally isolated from various countries produced sodium channel blocking (SCB) toxins, as ascertained by mouse neuroblastoma assay. The quantities of SCB toxins produced by bacteria and dinoflagellates were noted, and the limitations in comparing the toxicities of these two organisms are discussed. The chemical nature of the SCB toxins in selected bacterial isolates was determined as paralytic shellfish toxins by pre- and postcolumn high-performance liquid chromatography, capillary electrophoresis-mass spectrometry, and enzyme immunoassay.
引用
收藏
页码:239 / 245
页数:7
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