Agrobacterium-mediated high frequency transformation of an elite indica rice variety Pusa Basmati 1 and transmission of the transgenes to R2 progeny

被引：88

作者：

Mohanty, A

Sarma, NP

Tyagi, AK

机构：

[1] Univ Delhi, Ctr Plant Mol Biol, New Delhi 110021, India

[2] Univ Delhi, Dept Plant Mol Biol, New Delhi 110021, India

[3] Directorate Rice Res, Hyderabad 500030, Andhra Pradesh, India

来源：

PLANT SCIENCE | 1999年 / 147卷 / 02期

关键词：

Agrobacterium; gene expression; indica rice; inheritance; Oryza sativa L; transformation;

D O I：

10.1016/S0168-9452(99)00103-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among rice, indica varieties are considered recalcitrant to tissue culture and genetic manipulation. We report here Aglobactertim-mediated transformation of an economically important, elite indica variety Pusa Basmati 1. A large number of morphologically normal and fertile transgenic plants have been obtained. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgenes in the progeny of these plants. Twenty seven percent plants contain single copy gene insertion and copy number of transgenes has been found to vary from 1-4 in transgenic plants. Mendelian as well as non-Mendelian inheritance patterns of the introduced genes have been obtained in the R1 progeny. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：127 / 137

页数：11

共 37 条

[11] HERBICIDE-RESISTANT INDICA RICE PLANTS FROM IRRI BREEDING LINE IR72 AFTER PEG-MEDIATED TRANSFORMATION OF PROTOPLASTS [J].