Impaired signal transduction in neonatal platelets

被引:44
作者
Israels, SJ
Cheang, T
Roberston, C
McMillan-Ward, EM
McNicol, A
机构
[1] Univ Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada
[2] Univ Manitoba, Dept Pediat, Winnipeg, MB R3E 0V9, Canada
[3] Univ Manitoba, Dept Oral Biol, Winnipeg, MB R3E 0V9, Canada
关键词
D O I
10.1203/00006450-199905010-00014
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Previous in vitro studies of cord blood platelets from full-term and preterm neonates have demonstrated decreased responses to most physiologic agonists. This hyporesponsiveness is, in part, related to both deficient synthesis of, and response to, an important mediator of platelet function, thromboxane A(2)(TxA(2)). The poor response of neonatal platelets to TxA(2) is not due to differences in TxA(2) receptor binding characteristics, when compared with platelets from adult controls. Therefore, the postreceptor signal transduction pathway was investigated. The TxA(2) receptor is linked via the trimeric GTP-binding protein, Gq, to phospholipase C-beta (PLC beta), and stimulation of platelets with the stable TxA(2) mimetic, U46619, leads to activation of PLC beta and subsequent intracellular signaling events. U46619-induced P-32-phosphatidic acid formation, an index of PLC beta activation, was decreased in platelets of neonates (166 +/- 10%) when compared with adult controls (206 +/- 22%) (p < 0.05). Mobilization of intracellular calcium was impaired in platelets of newborns (175 +/- 49 nM) in comparison to adult controls (506 +/- 130 nM) (p < 0.01), after stimulation with U46619. U46619-stimulated GTPase activity was blunted in platelet membrane fractions from full-term neonates and almost absent in platelet membranes from preterm infants. Immunoblotting studies of the platelet membrane fractions, quantified by densitometric analysis, showed that levels of the G alpha q subunit were not significantly different between adult and neonate, and were not the cause of the marked differences in GTPase activity. These data suggest that signal transduction through the TxA(2) receptor is affected by decreased activity of Gq in platelets of neonates, and that this defect in signal transduction through PLC beta contributes to the observed poor response of newborns' platelets to TxA(2) and consequently to TxA(2)-dependent agonists such as collagen.
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收藏
页码:687 / 691
页数:5
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