Cephalosporinase over-expression resulting from insertion of ISAba1 in Acinetobacter baumannii

被引：231

作者：

Héritier, C ^{[1
]}

Poirel, L ^{[1
]}

Nordmann, P ^{[1
]}

机构：

[1] Univ Paris 11, Hop Bicetre, Assiatance Publ Hop Paris,Fac Med Paris Sud, Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2006年 / 12卷 / 02期

关键词：

Acinetobacter baumannii; ceftazidime resistance; cephalosporinase; insertion sequence; promoter sequence; ribosome binding site;

D O I：

10.1111/j.1469-0691.2005.01320.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

ISAba1-like sequences were identified immediately upstream of the bla(ampC) gene in ceftazidime-resistant Acinetobacter baumannii isolates, but were absent in ceftazidime-susceptible A. baumannii isolates. AmpC over-expression resulted from insertion of ISAba1-like sequences upstream of bla(ampC). ISAba1 provided strong promoter sequences, and it was demonstrated that the change in the ribosome binding site sequence resulting from insertion of ISAba1 did not influence expression of the bla(ampC) gene. Sequence analysis revealed that AmpC sequences of A. baumannii isolates were almost identical and that ISAba1 elements had a high percentage of identity.

引用

页码：123 / 130

页数：8

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