Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

被引：309

作者：

Alonso-Gutierrez, Jorge ^{[1
,2
]}

Chan, Rossana ^{[1
,2
]}

Batth, Tanveer S. ^{[1
,2
]}

Adams, Paul D. ^{[1
,2
]}

Keasling, Jay D. ^{[1
,2
,3
,4
]}

Petzold, Christopher J. ^{[1
,2
]}

Lee, Taek Soon ^{[1
,2
]}

机构：

[1] Joint Bioenergy Inst, Emeryville, CA 94608 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Chem & Biomol Engn, Berkeley, CA 94720 USA

来源：

METABOLIC ENGINEERING | 2013年 / 19卷

关键词：

Limonene; Perillyl alcohol; Mevalonate pathway; Microbial production; Metabolic engineering; Escherichia coli; HETEROLOGOUS MEVALONATE PATHWAY; GENE; PROTEIN; OPTIMIZATION; MONOTERPENES; EXPRESSION; TERPENOIDS; SYNTHASE; THERAPY; PLASMID;

D O I：

10.1016/j.ymben.2013.05.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (FOR) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce FOR. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400 mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100 mg/L of FOR. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. cob a potential production platform for any valuable limonene derivative. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：33 / 41

页数：9

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