Expression of Pancreatic Endocrine Markers by Mesenchymal Stem Cells From Human Adipose Tissue

被引：17

作者：

Silva, A. C. ^{[1
]}

Percegona, L. S. ^{[1
]}

Franca, A. L. ^{[1
]}

dos Santos, T. M. ^{[1
]}

Perini, C. C. ^{[1
]}

Gonzalez, P. ^{[1
]}

Rebelatto, C. L. K. ^{[1
]}

Camara, N. O. S. ^{[2
]}

Aita, C. A. M. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Parana, Ctr Ciencias Biol & Saude, BR-80215901 Curitiba, PR, Brazil

[2] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo, Brazil

来源：

TRANSPLANTATION PROCEEDINGS | 2012年 / 44卷 / 08期

关键词：

INSULIN; TRANSDIFFERENTIATION;

D O I：

10.1016/j.transproceed.2012.07.036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Mesenchymal stem cells (MSCs) from human adipose tissue have a great potential for use in cell therapy due to their ease of isolation, expansion, and differentiation, besides the relative acceptance from the ethical point of view. Our intention was to isolate and promote in vitro expansion and differentiation of MSCs from human adipose tissue into cells with a pancreatic endocrine phenotype. Human adipose tissue obtained from patients undergoing abdominal dermolipectomy was digested with type I collagenase. MSCs isolated by plastic adherence and characterized by cytochemistry and FACS were expanded in vitro. MSC differentiation into an endocrine phenotype was induced over 2 to 4 months with high glucose (25 mmol/L) media containing nicotinamide, exendin-4, and 2-mercaptoethanol. Insulin and glucagon expressions were analyzed by immunofluorescence. Cells isolated from human adipose tissue and expanded in vitro expressed MSC markers as confirmed by FACS and cytochemistry. Insulin but not glucagon production by differentiated cells was demonstrated by irnmunofluorescence. MSCs isolated from human adipose tissue were induced to differentiate in vitro into an endocrine phenotype that expressed insulin

引用

页码：2495 / 2496

页数：2

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