Production of Lewis x tetrasaccharides by metabolically engineered Escherichia coli

被引：45

作者：

Dumon, C ^{[1
]}

Bosso, C ^{[1
]}

Utille, JP ^{[1
]}

Heyraud, A ^{[1
]}

Samain, E ^{[1
]}

机构：

[1] UJF, CNRS,FR 2607, ICMG, Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 02期

关键词：

biosynthesis; carbohydrates; glycoconjugates; glycosyltransferases; metabolic engineering;

D O I：

10.1002/cbic.200500293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two tetrasaccharides carrying the trisaccharidic Lewis x motif on a GlcNAc or a Gal residue were produced on the gram-scale by high-cell-density cultures of metabolically engineered Escherichia coli strains that overexpressed the Helicobacter pylori futA gene for alpha-3 fucosyltransferase and the Neisseria meningitidis IgtB gene for beta-4 galactosyltransferase. The first compound Gal beta-4(Fuc alpha-3)GlcNAc beta-4GlcNAc was produced by glycosylation of chitinbiose, which was endogenously generated in the bacterial cytoplasm by the successive action of the rhizobial chitin-synthase NodC and the Bacillus circulans chitinase A1, whose genes were additionally expressed in the E. coli strain. The second compound, Gal beta-4(Fuc alpha-3)GlcNAc beta-3Gal, was produced from exogenously added Gal by a strain that was deficient in galactokinase activity and overexpressed the additional N. meningitidis lgtA gene for beta-3 N-acetylglucosominyltransferase.

引用

页码：359 / 365

页数：7

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