Co-transcriptional Assembly of Chemically Modified RNA Nanoparticles Functionalized with siRNAs

被引：108

作者：

Afonin, Kirill A. ^{[3
]}

Kireeva, Maria ^{[4
]}

Grabow, Wade W. ^{[1
]}

Kashlev, Mikhail ^{[4
]}

Jaeger, Luc ^{[1
,2
]}

Shapiro, Bruce A. ^{[3
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA

[3] NCI, Computat RNA Struct Grp, Ctr Canc Res, Nanobiol Program,Frederick Natl Lab Canc Res, Frederick, MD 21702 USA

[4] NCI, Gene Regulat & Chromosome Biol Lab, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA

来源：

NANO LETTERS | 2012年 / 12卷 / 10期

关键词：

RNA nanotechnology; self-assembly; siRNA; chemical modifications; transcription; T7 RNA polymerase; EMERGING FIELD; DESIGN; NANOTECHNOLOGY; POLYMERASE;

D O I：

10.1021/nl302302e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments.

引用

页码：5192 / 5195

页数：4

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