Self-Assembling RNA Nanorings Based on RNAI/II Inverse Kissing Complexes

被引：164

作者：

Grabow, Wade W. ^{[1
]}

Zakrevsky, Paul ^{[1
]}

Afonin, Kirill A. ^{[1
]}

Chworos, Arkadiusz ^{[1
,4
]}

Shapiro, Bruce A. ^{[2
]}

Jaeger, Luc ^{[1
,3
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[2] NCI, Ctr Canc Res, Nanobiol Program, NIH, Frederick, MD 21702 USA

[3] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA

[4] Polish Acad Sci, Ctr Mol & Macromol Studies, PL-90363 Lodz, Poland

来源：

NANO LETTERS | 2011年 / 11卷 / 02期

关键词：

RNA architectonics; bionanotechnology; silencing RNA; supramolecular assembly; RNA interaction; RNA motif; DIMERIZATION INITIATION SITE; APTAMER-SIRNA CHIMERAS; COLE1 ROM PROTEIN; CHEMICAL-MODIFICATION; TERTIARY INTERACTIONS; COMBINATORIAL RNAI; DNA NANOSTRUCTURES; LOOP COMPLEX; STEM-LOOPS; DESIGN;

D O I：

10.1021/nl104271s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

RNA is an attractive biopolymer for nanodesign of self-assembling particles for nanobiotechnology and synthetic biology. Here, we experimentally characterize by biochemical and biophysical methods the formation of thermostable and ribonuclease resistant RNA nanorings previously proposed by computational design. High yields of fully programmable nanorings were produced based on several RNAI/II kissing complex variants selected for their ability to promote polygon self-assembly. This self-assembly strategy relying on the particular geometry of bended kissing complexes has potential for developing short interfering RNA delivery agents.

引用

页码：878 / 887

页数：10

共 60 条

[1] Dumbbell-shaped nanocircular RNAs for RNA interference [J].

Abe, Naoko ;

Abe, Hiroshi ;

Ito, Yoshihiro .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (49) :15108-+

[2] Specific RNA self-assembly with minimal paranemic motifs [J].

Afonin, Kirill A. ;

Cieply, Dennis J. ;

Leontis, Neocles B. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (01) :93-102

[3]

Afonin KA, 2010, NAT NANOTECHNOL, V5, P676, DOI [10.1038/NNANO.2010.160, 10.1038/nnano.2010.160]

[4] RNA dynamics: it is about time [J].

Al-Hashimi, Hashim M. ;

Walter, Nils G. .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2008, 18 (03) :321-329

[5] Assembling materials with DNA as the guide [J].

Aldaye, Faisal A. ;

Palmer, Alison L. ;

Sleiman, Hanadi F. .

SCIENCE, 2008, 321 (5897) :1795-1799

[6] Chemical Modification of siRNAs for In Vivo Use [J].

Behlke, Mark A. .

OLIGONUCLEOTIDES, 2008, 18 (04) :305-319

[7] Computational strategies for the automated design of RNA nanoscale structures from building blocks using NanoTiler [J].

Bindewald, Eckart ;

Grunewald, Calvin ;

Boyle, Brett ;

O'Connor, Mary ;

Shapiro, Bruce A. .

JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 2008, 27 (03) :299-308

[8] RNAJunction: a database of RNA junctions and kissing loops for three-dimensional structural analysis and nanodesign [J].

Bindewald, Eckart ;

Hayes, Robert ;

Yingling, Yaroslava G. ;

Kasprzak, Wojciech ;

Shapiro, Bruce A. .

NUCLEIC ACIDS RESEARCH, 2008, 36 :D392-D397

[9] RNA loop-loop interactions as dynamic functional motifs [J].

Brunel, C ;

Marquet, R ;

Romby, P ;

Ehresmann, C .

BIOCHIMIE, 2002, 84 (09) :925-944

[10] The promises and pitfalls of RNA-interference-based therapeutics [J].

Castanotto, Daniela ;

Rossi, John J. .

NATURE, 2009, 457 (7228) :426-433

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