Enzyme immobilization on porous silicon surfaces

被引：100

作者：

Létant, SE

Hart, BR

Kane, SR

Hadi, MZ

Shields, SJ

Reynolds, JG

机构：

[1] Lawrence Livermore Natl Lab, Livermore, CA 94551 USA

[2] Lockheed Martin Corp, Sandia Natl Labs, Livermore, CA 94551 USA

来源：

ADVANCED MATERIALS | 2004年 / 16卷 / 08期

关键词：

D O I：

10.1002/adma.200306173

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

beta-Glucuronidase enzymes have been attached to a porous silicon surface through a direct silicon-carbon bond based linking system (see Figure). The attached enzymes display high activity and the photoluminescent (PL) properties and surface stability of the porous silicon are retained. Quenching of the PL is observed upon enzymatic breakdown of the substrate, leading to the possibility of a new class of chemical and biological sensors.

引用

页码：689 / +

页数：6

共 25 条

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