Combination of chromatographic and spectroscopic methods for the isolation and characterization of polar guaianolides from Achillea asiatica

被引：9

作者：

Glasl, S

Gunbilig, D

Samdan, N

Werner, I

Jurenitsch, J

机构：

[1] Univ Vienna, Inst Pharmacognosy, Ctr Pharm, A-1090 Vienna, Austria

[2] Mongolian Acad Sci, Inst Chem & Chem Technol, Ulaanbaatar, Mongolia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 936卷 / 1-2期

关键词：

Achillea asiatica; plant materials; guaianolides; sesquiterpenoids;

D O I：

10.1016/S0021-9673(01)00947-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four polar guaianolides, 8 alpha -angeloxy-2 alpha ,4 alpha ,10 beta -trihydroxy-6 betaH,7 alphaH,11 betaH-1(5)-guaien-12,6 alpha -olide; 8 alpha -angeloxy-1 beta ,2 beta :4 beta ,5 beta -diepoxy-10 beta -hydroxy-6 betaH,7 alphaH,11 betaH-12,6 alpha -guaianolide; 8 alpha -angeloxy-4 alpha ,10 beta -dihydroxy-2-oxo-6 betaH,7 alphaH,11 betaH-1(5)-guaien-12,6 alpha -olide and 8-desacetyl-matrticarin, were isolated from Achillea asiatica and characterized by TLC, MS, IR, HPLC and diode array detection. Purified extracts were separated by means of flash chromatography. HPLC separations were achieved using different methanol-water gradients as mobile phase and LiChrospher 100-RP8 5 mum or Zorbax SB-C-8 3.5 mum as stationary phases. The chromatographical data are compared to those of the proazulene 8 alpha -tigloxy-artabsin which shows antiinflammatory effects. By means of these characteristics the identification of the guaianolides with potential antiphlogistic properties is also possible from other sources. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：193 / 200

页数：8

共 17 条

[1]

Della Loggia R., 1992, Planta Medica, V58, pA641, DOI 10.1055/s-2006-961648

[2] Qualitative and quantitative determination of sesquiterpenoids in Achillea species by reversed-phase high-performance liquid chromatography, mass-spectrometry and thin-layer chromatography [J].