The preparation of amperometric xanthine sensors based on multilayer thin films containing xanthine oxidase

被引：21

作者：

Hoshi, T ^{[1
]}

Noguchi, T ^{[1
]}

Anzai, J ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2006年 / 26卷 / 01期

关键词：

xanthine biosensor; xanthine oxidase; polyelectrolyte multilayer film; Pt electrode;

D O I：

10.1016/j.msec.2005.06.001

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

An amperometric xanthine biosensor was prepared by immobilizing xanthine oxidase (XOx) on the surface of a platinum (Pt) disk electrode. The Pt electrode was first covered with a polyelectrolyte multilayer (PEM) film composed of poly(allylamine hydrochloride) (PAH) and poly(vinyl sulfate) (PVS) to block the Pt surface from the access of uric acid, a reaction product of the XOx-catalyzed oxidation reaction of xanthine, because uric acid can be electrochemically oxidized on the Pt electrode to induce interference. The PEM film-covered Pt electrode was further modified with a XOx-containing multilayer film composed of XOx and poly(dimethyldiallylammonium chloride) (PDDA). The xanthine biosensors thus prepared can be used successfully for detecting xanthine in the concentration range of 3-300 mu M. The output current of the sensors depended on the number of the XOx/PDDA layers in the film. The PAH/PVS layer was effective to eliminate the uric acid interference. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：100 / 103

页数：4

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