A complete neandertal mitochondrial genome sequence determined by high-throughput Sequencing

被引：379

作者：

Green, Richard E. ^{[1
]}

Malaspinas, Anna-Sapfo ^{[2
]}

Krause, Johannes ^{[1
]}

Briggs, Adrian W. ^{[1
]}

Johnson, Philip L. F. ^{[3
]}

Uhler, Caroline ^{[4
]}

Meyer, Matthias ^{[1
]}

Good, Jeffrey M. ^{[1
]}

Maricic, Tomislav ^{[1
]}

Stenzel, Udo ^{[1
]}

Pruefer, Kay ^{[1
]}

Siebauer, Michael ^{[1
]}

Burbano, Hernan A. ^{[1
]}

Ronan, Michael ^{[5
]}

Rothberg, Jonathan M. ^{[6
]}

Egholm, Michael ^{[5
]}

Rudan, Pavao

Brajkovic, Dejana ^{[7
]}

Kucan, Zeljko

Gusic, Ivan

Wikstrom, Marten ^{[8
]}

Laakkonen, Liisa ^{[9
]}

Kelso, Janet ^{[1
]}

Slatkin, Montgomery ^{[2
]}

Paeaebo, Svante ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany

[2] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Stat, Berkeley, CA 94720 USA

[5] 454 Life Sci, Branford, CT 06405 USA

[6] Rothberg Inst Childhood Dis, Guilford, CT 06437 USA

[7] Croatian Acad Sci & Arts, Inst Quaternary Paleontol & Geol, HR-10000 Zagreb, Croatia

[8] Univ Helsinki, Inst Biotechnol, Program Struct Biol & Biophys, Helsinki Bioenergent Grp, FIN-00014 Helsinki, Finland

[9] Univ Helsinki, Fac Biosci, Dept Biol & Environm Sci, Div Biochem, FIN-00014 Helsinki, Finland

来源：

CELL | 2008年 / 134卷 / 03期

关键词：

D O I：

10.1016/j.cell.2008.06.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complete mitochondrial (mt) genome sequence was reconstructed from a 38,000 year-old Neandertal individual with 8341 mtDNA sequences identified among 4.8 Gb of DNA generated from similar to 0.3 g of bone. Analysis of the assembled sequence unequivocally establishes that the Neandertal mtDNA falls outside the variation of extant human mtDNAs, and allows an estimate of the divergence date between the two mtDNA lineages of 660,000 +/- 140,000 years. Of the 13 proteins encoded in the mtDNA, subunit 2 of cytochrome c oxidase of the mitochondrial electron transport chain has experienced the largest number of amino acid substitutions in human ancestors since the separation from Neandertals. There is evidence that purifying selection in the Neandertal mtDNA was reduced compared with other primate lineages, suggesting that the effective population size of Neandertals was small.

引用

页码：416 / 426

页数：11

共 58 条

[1] Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA [J].

Andrews, RM ;

Kubacka, I ;

Chinnery, PF ;

Lightowlers, RN ;

Turnbull, DM ;

Howell, N .

NATURE GENETICS, 1999, 23 (02) :147-147

[2] Proton-coupled electron transfer drives the proton pump of cytochrome c oxidase [J].

Belevich, I ;

Verkhovsky, MI ;

Wikström, M .

NATURE, 2006, 440 (7085) :829-832

[3] Patterns of damage in genomic DNA sequences from a Neandertal [J].

Briggs, Adrian W. ;

Stenzel, Udo ;

Johnson, Philip L. F. ;

Green, Richard E. ;

Kelso, Janet ;

Pruefer, Kay ;

Meyer, Matthias ;

Krause, Johannes ;

Ronan, Michael T. ;

Lachmann, Michael ;

Paeaebo, Svante .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (37) :14616-14621

[4] Novel high-resolution characterization of ancient DNA reveals C>U-type base modification events as the sole cause of post mortem miscoding lesions [J].

Brotherton, Paul ;

Endicott, Phillip ;

Sanchez, Juan J. ;

Beaumont, Mark ;

Barnett, Ross ;

Austin, Jeremy ;

Cooper, Alan .

NUCLEIC ACIDS RESEARCH, 2007, 35 (17) :5717-5728

[5] A new hominid from the Upper Miocene of Chad, central Africa [J].

Brunet, M ;

Guy, F ;

Pilbeam, D ;

Mackaye, HT ;

Likius, A ;

Ahounta, D ;

Beauvilain, A ;

Blondel, C ;

Bocherens, H ;

Boisserie, JR ;

De Bonis, L ;

Coppens, Y ;

Dejax, J ;

Denys, C ;

Duringer, P ;

Eisenmann, VR ;

Fanone, G ;

Fronty, P ;

Geraads, D ;

Lehmann, T ;

Lihoreau, F ;

Louchart, A ;

Mahamat, A ;

Merceron, G ;

Mouchelin, G ;

Otero, O ;

Campomanes, PP ;

De Leon, MP ;

Rage, JC ;

Sapanet, M ;

Schuster, M ;

Sudre, J ;

Tassy, P ;

Valentin, X ;

Vignaud, P ;

Viriot, L ;

Zazzo, A ;

Zollikofer, C .

NATURE, 2002, 418 (6894) :145-151

[6] Modern humans did not admix with Neanderthals during their range expansion into Europe [J].

Currat, M ;

Excoffier, L .

PLOS BIOLOGY, 2004, 2 (12) :2264-2274

[7] Coadaptive evolution in cytochrome c oxidase:: 9 of 13 subunits show accelerated rates of nonsynonymous substitution in anthropoid primates [J].

Doan, JW ;

Schmidt, TR ;

Wildman, DE ;

Uddin, M ;

Goldberg, A ;

Hüttemann, M ;

Goodman, M ;

Weiss, ML ;

Grossman, LI .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 2004, 33 (03) :944-950

[8] Comparative Genomics and the evolution of human mitochondrial DNA: Assessing the effects of selection [J].

Elson, JL ;

Turnbull, DM ;

Howell, N .

AMERICAN JOURNAL OF HUMAN GENETICS, 2004, 74 (02) :229-238

[9] External and internal morphology of the BAR 1002′00 Orrorin tugenensis femur [J].

Galik, K ;

Senut, B ;

Pickford, M ;

Gommery, D ;

Treil, J ;

Kuperavage, AJ ;

Eckhardt, RB .

SCIENCE, 2004, 305 (5689) :1450-1453

[10] Whole-genome shotgun sequencing of mitochondria from ancient hair shafts [J].

Gilbert, M. Thomas P. ;

Tomsho, Lynn P. ;

Rendulic, Snjezana ;

Packard, Michael ;

Drautz, Daniela I. ;

Sher, Andrei ;

Tikhonov, Alexei ;

Dalen, Love ;

Kuznetsova, Tatyana ;

Kosintsev, Pavel ;

Campos, Paula F. ;

Higham, Thomas ;

Collins, Matthew J. ;

Wilson, Andrew S. ;

Shidlovskiy, Fyodor ;

Buigues, Bernard ;

Ericson, Per G. P. ;

Germonpre, Mietje ;

Goetherstroem, Anders ;

Iacumin, Paola ;

Nikolaev, Vladimir ;

Nowak-Kemp, Malgosia ;

Willerslev, Eske ;

Knight, James R. ;

Irzyk, Gerard P. ;

Perbost, Clotilde S. ;

Fredrikson, Karin M. ;

Harkins, Timothy T. ;

Sheridan, Sharon ;

Miller, Webb ;

Schuster, Stephan C. .

SCIENCE, 2007, 317 (5846) :1927-1930

← 1 2 3 4 5 6 →