Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

被引：192

作者：

Gilbert, M. Thomas P.

Tomsho, Lynn P.

Rendulic, Snjezana

Packard, Michael

Drautz, Daniela I.

Sher, Andrei

Tikhonov, Alexei

Dalen, Love

Kuznetsova, Tatyana

Kosintsev, Pavel

Campos, Paula F.

Higham, Thomas

Collins, Matthew J.

Wilson, Andrew S.

Shidlovskiy, Fyodor

Buigues, Bernard

Ericson, Per G. P.

Germonpre, Mietje

Goetherstroem, Anders

Iacumin, Paola

Nikolaev, Vladimir

Nowak-Kemp, Malgosia

Willerslev, Eske

Knight, James R.

Irzyk, Gerard P.

Perbost, Clotilde S.

Fredrikson, Karin M.

Harkins, Timothy T.

Sheridan, Sharon

Miller, Webb

Schuster, Stephan C.

机构：

[1] Penn State Univ, Ctr Comparat Genom & Bioinformat, University Pk, PA 16802 USA

[2] Univ Copenhagen, Ctr Ancient Genet, DK-2100 Copenhagen, Denmark

[3] Russian Acad Sci, Severtsov Inst Ecol & Evolut, Moscow 119071, Russia

[4] Russian Acad Sci, Inst Zool, St Petersburg 199034, Russia

[5] ISCII Evoluc & Comportamiento Humanos, Ctr UCM, Madrid 28029, Spain

[6] Moscow MV Lomonosov State Univ, Fac Geol, Dept Paleontol, Moscow 119992, Russia

[7] Russian Acad Sci, Urals Branch, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia

[8] Univ Oxford Archaeol & Hist Art Res Lab, Oxford OX1 3QY, England

[9] Ice Age Museum, All Russia Exhibit Ctr, York YO10 5YW, N Yorkshire, England

[10] Swedish Museum Nat Hist, Dept Vertebrate Zool, S-10405 Stockholm, Sweden

[11] Inst Royal Sci Nat Belgique, Dept Palaeontol, B-1000 Brussels, Belgium

[12] Uppsala Univ, Evolutionary Biol Ctr, Dept Evolutionary Biol, SE-75236 Uppsala, Sweden

[13] Univ Parma, Dept Earth Sci, I-43100 Parma, Italy

[14] Russian Acad Sci, Inst Geog, Dept Glaciol, Moscow 109017, Russia

[15] Univ Oxford, Museum Nat Hist, Oxford OX1 3PW, England

[16] Roche Diagnost Corp, Indianapolis, IN 46250 USA

来源：

SCIENCE | 2007年 / 317卷 / 5846期

基金：

英国自然环境研究理事会;

关键词：

D O I：

10.1126/science.1146971

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 C-14 years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

引用

页码：1927 / 1930

页数：4

共 25 条

[1] Genetic structure and extinction of the woolly mammoth, Mammuthus primigenius [J].