Increased production of eicosapentaenoic acid by Skeletonema costatum cells after decantation at low temperature

被引：30

作者：

Blanchemain, A ^{[1
]}

Grizeau, D ^{[1
]}

机构：

[1] Conservatoire Natl Arts & Metiers, Inst Natl Sci & Tech Mer, Equipe Biotechnol Marines, F-50103 Cherbourg, France

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 07期

关键词：

eicosapentaenoic acid; fatty acids; microalgal; Skeletonema costatum;

D O I：

10.1023/A:1008989730798

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coagulation of Skeletonema costatum biomass was induced at alkaline pH in enriched sea-water to concentrate the microalgal suspensions by decantation. Incubation of the concentrated cultures at 15 degrees C after return at pH 7.5 preserved cell viability and increased the yield of lipid extraction and the 20:5(n-3) fatty acid content of the diatom biomass.

引用

收藏

页码：497 / 501

页数：5

相关论文

共 13 条

[1] ORIGIN OF MARINE FATTY ACIDS .1. ANALYSES OF THE FATTY ACIDS PRODUCED BY THE DIATOM SKELETONEMA-COSTATUM [J].

ACKMAN, RG ;

JANGAARD, PM ;

HOYLE, RJ ;

BROCKERHOFF, H .

JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1964, 21 (04) :747-756

[2] Eicosapentaenoic acid content of Skeletonema costatum as a function of growth and irradiance; Relation with chlorophyll a content and photosynthetic capacity [J].

Blanchemain, A ;

Grizeau, D .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1996, 196 (1-2) :177-188

[3] EFFECT OF DIFFERENT ORGANIC BUFFERS ON THE GROWTH OF SKELETONEMA-COSTATUM CULTURES - FURTHER EVIDENCE FOR AN AUTOINHIBITORY EFFECT [J].

BLANCHEMAIN, A ;

GRIZEAU, D ;

GUARY, JC .

JOURNAL OF PLANKTON RESEARCH, 1994, 16 (10) :1433-1440

[4]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[5]

Christie W., 1989, GAS CHROMATOGRAPHY L

[6] INCREASE OF LIPID YIELDS FROM SOME ALGAE BY ACID-EXTRACTION [J].

DUBINSKY, Z ;

AARONSON, S .

PHYTOCHEMISTRY, 1979, 18 (01) :51-52

[7] FATTY-ACID AND ELEMENTAL COMPOSITION OF THE MARINE DIATOM CHAETOCEROS-GRACILIS SCHUTT - EFFECTS OF SILICATE DEPRIVATION, TEMPERATURE AND LIGHT-INTENSITY [J].

MORTENSEN, SH ;

BORSHEIM, KY ;

RAINUZZO, JR ;

KNUTSEN, G .

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 1988, 122 (02) :173-185

[8] Effect of temperature on the growth, total lipid content and fatty acid composition of recently isolated tropical microalgae Isochrysis sp, Nitzschia closterium, Nitzschia paleacea, and commercial species Isochrysis sp (clone T ISO) [J].

Renaud, SM ;

Zhou, HC ;

Parry, DL ;

Thinh, LV ;

Woo, KC .

JOURNAL OF APPLIED PHYCOLOGY, 1995, 7 (06) :595-602

[9] CULTURE CONDITIONS AFFECT EICOSAPENTAENOIC ACID CONTENT OF CHLORELLA-MINUTISSIMA [J].

SETO, A ;

WANG, HL ;

HESSELTINE, CW .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1984, 61 (05) :892-894

[10] EFFECTS OF VARIATION IN TEMPERATURE .2. ON THE FATTY-ACID COMPOSITION OF 8 SPECIES OF MARINE-PHYTOPLANKTON [J].

THOMPSON, PA ;

GUO, MX ;

HARRISON, PJ ;

WHYTE, JNC .

JOURNAL OF PHYCOLOGY, 1992, 28 (04) :488-497