Identification of a Stat gene that functions in Drosophila development

被引:332
作者
Yan, RQ
Small, S
Desplan, C
Dearolf, CR
Darnell, JE
机构
[1] NYU,DEPT BIOL,NEW YORK,NY 10003
[2] ROCKEFELLER UNIV,HOWARD HUGHES MED INST,NEW YORK,NY 10021
[3] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEV GENET GRP,BOSTON,MA 02115
[4] HARVARD UNIV,SCH MED,CANC BIOL SECT,JOINT CTR RADIAT THERAPY,BOSTON,MA 02115
关键词
D O I
10.1016/S0092-8674(00)81287-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified. This protein becomes phosphorylated on Tyr-704 when coexpressed in Schneider cells with a Drosophila janus kinase (JAK), Hopscotch (HOP). The phosphorylated protein binds specifically to the consensus sequence TTCCCGGAA. Suppressor mutations of hop(Tum-1), a dominant hyperactive allele of hop whose phenotype is hematocyte overproduction and tumor formation, were selected. One of these mutants, stat(HJ), mapped to the same chromosomal region (92E) as does D-Stat, had an incompletely penetrant pair rule phenotype, and exhibited aberrant expression of the pair rule gene even skipped (eve) at the cellular blastoderm stage. Two D-STAT-binding sites were identified within the eve stripe 3 enhancer region. Mutations in either of the STAT-binding sites greatly decreased the stripe 3 expression in transgenic flies. Clearly, the JAK-STAT pathway is connected to Drosophila early development.
引用
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页码:421 / 430
页数:10
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