Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes

被引：32

作者：

Braun, L ^{[1
]}

Csala, M ^{[1
]}

Poussu, A ^{[1
]}

Garzo, T ^{[1
]}

Mandl, J ^{[1
]}

Banhegyi, G ^{[1
]}

机构：

[1] SEMMELWEIS UNIV MED, DEPT MED CHEM, H-1444 BUDAPEST, HUNGARY

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

基金：

匈牙利科学研究基金会;

关键词：

glutathione; glycogenolysis; ascorbate; mouse hepatocyte;

D O I：

10.1016/0014-5793(96)00548-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonolactone could not be further affected by glutathione depletion. Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption, Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.

引用

页码：173 / 176

页数：4

共 40 条

[1] ESTIMATION AND IDENTIFICATION OF THIOLS IN RAT SPLEEN AFTER CYSTEINE OR GLUTATHIONE TREATMENT - RELEVANCE TO PROTECTION AGAINST NITROGEN MUSTARDS [J].

BALL, CR .

BIOCHEMICAL PHARMACOLOGY, 1966, 15 (07) :809-&

[2] Ascorbate synthesis-dependent glutathione consumption in mouse liver [J].

Banhegyi, G ;

Csala, M ;

Braun, L ;

Garzo, T ;

Mandl, J .

FEBS LETTERS, 1996, 381 (1-2) :39-41

[3] GLYCOGENOLYSIS - AND NOT GLUCONEOGENESIS - IS THE SOURCE OF UDP-GLUCURONIC ACID FOR GLUCURONIDATION [J].

BANHEGYI, G ;

GARZO, T ;

ANTONI, F ;

MANDL, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 967 (03) :429-435

[4] LATENCY IS THE MAJOR DETERMINANT OF UDP-GLUCURONOSYLTRANSFERASE ACTIVITY IN ISOLATED HEPATOCYTES [J].

BANHEGYI, G ;

GARZO, T ;

FULCERI, R ;

BENEDETTI, A ;

MANDL, J .

FEBS LETTERS, 1993, 328 (1-2) :149-152

[5] INCREASE IN CYTOSOLIC CA-2+ CONCENTRATION DURING TERT-BUTYL HYDROPEROXIDE METABOLISM BY ISOLATED HEPATOCYTES INVOLVES NADPH OXIDATION AND MOBILIZATION OF INTRACELLULAR CA-2+ STORES [J].

BELLOMO, G ;

THOR, H ;

ORRENIUS, S .

FEBS LETTERS, 1984, 168 (01) :38-42

[6] ASCORBIC-ACID SYNTHESIS IS STIMULATED BY ENHANCED GLYCOGENOLYSIS IN MURINE LIVER [J].

BRAUN, L ;

GARZO, T ;

MANDL, J ;

BANHEGYI, G .

FEBS LETTERS, 1994, 352 (01) :4-6

[7] IDENTIFICATION AND QUANTITATION OF GLUTATHIONE IN HEPATIC PROTEIN MIXED DISULFIDES AND ITS RELATIONSHIP TO GLUTATHIONE DISULFIDE [J].

BRIGELIUS, R ;

MUCKEL, C ;

AKERBOOM, TPM ;

SIES, H .

BIOCHEMICAL PHARMACOLOGY, 1983, 32 (17) :2529-2534

[8] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

BURTON, K .

BIOCHEMICAL JOURNAL, 1956, 62 (02) :315-323

[9] ALTERATIONS IN INTRACELLULAR THIOL HOMEOSTASIS DURING THE METABOLISM OF MENADIONE BY ISOLATED RAT HEPATOCYTES [J].

DIMONTE, D ;

ROSS, D ;

BELLOMO, G ;

EKLOW, L ;

ORRENIUS, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1984, 235 (02) :334-342

[10]

ERNEST MJ, 1973, J BIOL CHEM, V248, P1550

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