Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes

被引：32

作者：

Braun, L ^{[1
]}

Csala, M ^{[1
]}

Poussu, A ^{[1
]}

Garzo, T ^{[1
]}

Mandl, J ^{[1
]}

Banhegyi, G ^{[1
]}

机构：

[1] SEMMELWEIS UNIV MED, DEPT MED CHEM, H-1444 BUDAPEST, HUNGARY

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

基金：

匈牙利科学研究基金会;

关键词：

glutathione; glycogenolysis; ascorbate; mouse hepatocyte;

D O I：

10.1016/0014-5793(96)00548-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonolactone could not be further affected by glutathione depletion. Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption, Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.

引用

页码：173 / 176

页数：4

共 40 条

[11]

ERNEST MJ, 1974, J BIOL CHEM, V249, P5011

[12]

FAIN JN, 1971, MOL PHARMACOL, V7, P465

[13]

Griffith O W, 1981, Methods Enzymol, V77, P59

[14] DECREASE OF GLUTATHIONE AND INDUCTION OF GAMMA-GLUTAMYLTRANSFERASE BY DIBUTYRYL-3', 5'-CYCLIC AMP IN RAT-LIVER [J].

HIGASHI, T ;

TATEISHI, N ;

NARUSE, A ;

SAKAMOTO, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 68 (04) :1280-1286

[15] RAPID SPECIFIC METHOD FOR DETERMINATION OF ALDOSACCHARIDES IN BODY FLUIDS [J].

HULTMAN, E .

NATURE, 1959, 183 (4654) :108-109

[16]

JEWELL SA, 1982, SCIENCE, V217, P1257, DOI 10.1126/science.7112127

[17] DIAMIDE, A NEW REAGENT FOR INTRACELLULAR OXIDATION OF GLUTATHIONE TO DISULFIDE [J].

KOSOWER, NS ;

KOSOWER, EM ;

WERTHEIM, B ;

CORREA, WS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 37 (04) :593-&

[18]

LAU KHW, 1983, J BIOL CHEM, V258, P2321

[19]

LEVINE M, 1986, NEW ENGL J MED, V314, P892, DOI 10.1056/NEJM198604033141407

[20] EVALUATION OF THE CALCIUM MOBILIZING ACTION OF ACETAMINOPHEN AND BROMOBENZENE IN RAT HEPATOCYTE CULTURES [J].

LONG, RM ;

MOORE, L .

JOURNAL OF BIOCHEMICAL TOXICOLOGY, 1988, 3 :353-362

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