Gibberellin biosynthesis in bacteria: Separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum

被引：125

作者：

Morrone, Dana ^{[1
]}

Chambers, Jacob ^{[2
]}

Lowry, Luke ^{[1
]}

Kim, Gunjune ^{[3
]}

Anterola, Aldwin ^{[3
]}

Bender, Kelly ^{[2
]}

Peters, Reuben J. ^{[1
]}

机构：

[1] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

[2] So Illinois Univ, Dept Microbiol, Carbondale, IL 62901 USA

[3] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA

来源：

FEBS LETTERS | 2009年 / 583卷 / 02期

基金：

美国国家科学基金会;

关键词：

Copalyl diphosphate synthase; ent-Kaurene synthase; Gibberellin; Diterpene; Terpene synthase; Bradyrhizobium japonicum; BIFUNCTIONAL DITERPENE CYCLASE; FUNCTIONAL-ANALYSIS; AZOSPIRILLUM-LIPOFERUM; GENE-CLUSTER; INITIATED CYCLIZATION; FUNGI; CDNA; IDENTIFICATION; METABOLISM; FUJIKUROI;

D O I：

10.1016/j.febslet.2008.12.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional diterpene synthase. We demonstrate here that Bradyrhizobium japonicum encodes separate ent-copalyl diphosphate and ent-kaurene synthases. These are found in an operon whose enzymatic composition indicates that gibberellin biosynthesis in bacteria represents a third independently assembled pathway relative to plants and fungi. Nevertheless, sequence comparisons also suggest potential homology between diterpene synthases from bacteria, plants, and fungi. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：475 / 480

页数：6