Positional cloning of the mouse circadian Clock gene

被引：1124

作者：

King, DP ^{[1
]}

Zhao, YL ^{[1
]}

Sangoram, AM ^{[1
]}

Wilsbacher, LD ^{[1
]}

Tanaka, M ^{[1
]}

Antoch, MP ^{[1
]}

Steeves, TDL ^{[1
]}

Vitaterna, MH ^{[1
]}

Kornhauser, JM ^{[1
]}

Lowrey, PL ^{[1
]}

Turek, FW ^{[1
]}

Takahashi, JS ^{[1
]}

机构：

[1] NORTHWESTERN UNIV, DEPT NEUROBIOL & PHYSIOL, NATL SCI FDN CTR BIOL TIMING, EVANSTON, IL 60208 USA

来源：

CELL | 1997年 / 89卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(00)80245-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used positional cloning to identify the circadian Clock gene in mice. Clock is a large transcription unit with 24 exons spanning similar to 100,000 bp of DNA from which transcript classes of 7.5 and similar to 10 kb arise. Clock encodes a novel member of the bHLH-PAS family of transcription factors. In the Clock mutant allele, an A-->T nucleotide transversion in a splice donor site causes exon skipping and deletion of 51 amino acids in the CLOCK protein. Clock is a unique gene with known circadian function and with features predicting DNA binding, protein dimerization, and activation domains. CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism.

引用

页码：641 / 653

页数：13

共 77 条

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