A convenient set of vectors for expression of multiple gene combinations in plants

被引：5

作者：

deMajnik, J

Joseph, RG

Tanner, GJ

Larkin, PJ

Djordjevic, MA

Rolfe, BG

Weinman, JJ

机构：

[1] AUSTRALIAN NATL UNIV,RES SCH BIOL SCI,PLANT MICROBE INTERACT GRP,CANBERRA,ACT 2601,AUSTRALIA

[2] CSIRO,DIV PLANT IND,CANBERRA,ACT 2601,AUSTRALIA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1997年 / 15卷 / 02期

关键词：

anthocyanin; expression cassette; transformation; transient expression; vector;

D O I：

10.1007/BF02812263

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of vectors has been developed that simplify shuttling expression cassettes between small plasmids of high copy number ideal for experiments involving biolistic transient expression and a binary transformation plasmid. Three cassettes for the expression of a cloned coding sequence behind different promoters have been modified; combinations of these cassettes can be excised with Not I, and sequentially cloned into the transformation vector in a procedure that removes the first cloning site. The system is demonstrated by inducing anthocyanin synthesis with paired regulatory genes of maize biolistically delivered to a maize cell suspension, and then expressed in transformed tobacco.

引用

页码：134 / 140

页数：7

共 11 条

[1] REGULATED TRANSCRIPTION OF THE MAIZE BRONZE-2 PROMOTER IN ELECTROPORATED PROTOPLASTS REQUIRES THE C1 AND R GENE-PRODUCTS [J].