Real-time PCR for detection of Brucella spp. DNA in human serum samples

被引：52

作者：

Debeaumont, C ^{[1
]}

Falconnet, PA ^{[1
]}

Maurin, M ^{[1
]}

机构：

[1] Univ Grenoble 1, Bacteriol Lab, CHU, Fac Med, F-38043 Grenoble 9, France

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2005年 / 24卷 / 12期

关键词：

D O I：

10.1007/s10096-005-0064-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Presented here are the results of an evaluation of an in-house real-time PCR assay for the rapid and specific diagnosis of human brucellosis. The assay was based on direct amplification from serum samples of a 169-bp portion of bcsp31, a gene found in all Brucella species and biovars. Species specificity and selectivity of this real-time PCR assay were evaluated using genomic DNA from 15 Brucella strains and 42 non-Brucella strains, and the results were 100%. Among 17 culture-proven brucellosis patients, sera from 11 gave a positive amplification signal, corresponding to a sensitivity of 64.7%. In contrast, negative results were obtained for all sera from 60 control patients, corresponding to a specificity of 100%. The results indicate this test is well adapted for definite confirmation of brucellosis cases, when Brucella cultures remain sterile and serological tests demonstrate the presence of cross-reacting antibodies against Brucella sp. and Yersinia enterocolitica O:9 antigens.

引用

页码：842 / 845

页数：4

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