Modification of Lowry's method for collagen concentration measurement

被引：67

作者：

KomsaPenkova, R ^{[1
]}

Spirova, R ^{[1
]}

Bechev, B ^{[1
]}

机构：

[1] MED UNIV PLEVEN,DEPT BIOPHYS,BU-5000 PLEVEN,BULGARIA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 32卷 / 01期

关键词：

method; collagen concentration measurement; collagen type; gelatine;

D O I：

10.1016/0165-022X(95)00046-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the determination of collagen concentration is described, It is simple and sensitive and is based on a modification of Lowry's method. The modification includes an initial heating of collagen samples in alkaline solution and changes in the concentrations of reagents used, It may be used for different collagen types, i.e. I, II, V and XI, from various sources as well as for gelatine. The influence of different buffers, salts, detergents, etc. on the precision of the method was studied. The precision of the measurements was not influenced by pH, the turbidity of the sample ana the presence of certain salts, osmolytes and preservatives, routinely used in biochemical, microbiological and immunological experimental protocols. The modification is suitable for routine determinations of collagen concentration in a wide variety of samples.

引用

页码：33 / 43

页数：11

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