Isolation and characterization of a mouse SRY-related cDNA, mSox7

被引：38

作者：

Taniguchi, K

Hiraoka, Y

Ogawa, M

Sakai, Y

Kido, S

Aiso, S

机构：

[1] Keio Univ, Sch Med, Dept Anat, Shinjuku Ku, Tokyo 1608582, Japan

[2] Tokyo Elect Power Hosp, Shinjuku Ku, Tokyo 1600016, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1445卷 / 02期

关键词：

DNA binding; HMG box; oocyte; SOX; SRY;

D O I：

10.1016/S0167-4781(99)00047-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SOX is a family of SR Y-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：225 / 231

页数：7

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