Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells

被引：19

作者：

Chen, Guan-Yu ^{[1
]}

Li, Zeyang ^{[2
]}

Theile, Christopher S. ^{[1
]}

Bardhan, Neelkanth M. ^{[3
]}

Kumar, Priyank V. ^{[4
]}

Duarte, Joao N. ^{[1
]}

Maruyama, Takeshi ^{[1
]}

Rashidfarrokh, Ali ^{[1
]}

Belcher, Angela M. ^{[5
]}

Ploegh, Hidde L. ^{[6
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Whitehead Inst Biomed Res, Dept Chem, Cambridge, MA 02139 USA

[3] MIT, David H Koch Inst Integrat Canc Res, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[4] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[5] MIT, David H Koch Inst Integrat Canc Res, Dept Biol Engn, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[6] MIT, Whitehead Inst Biomed Res, Dept Biol, Cambridge, MA 02142 USA

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2015年 / 21卷 / 48期

基金：

美国国家卫生研究院;

关键词：

blood; cell detection; graphene oxide; single domain antibody; sortase; CIRCULATING TUMOR-CELLS; ORIENTATION; SENSITIVITY; PHENOTYPE; RELEASE;

D O I：

10.1002/chem.201503057

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Peripheral blood can provide valuable information on an individual's immune status. Cell-based assays typically target leukocytes and their products. Characterization of leukocytes from whole blood requires their separation from the far more numerous red blood cells.([1]) Current methods to classify leukocytes, such as recovery on antibody-coated beads or fluorescence-activated cell sorting require long sample preparation times and relatively large sample volumes.([2]) A simple method that enables the characterization of cells from a small peripheral whole blood sample could overcome limitations of current analytical techniques. We describe the development of a simple graphene oxide surface coated with single-domain antibody fragments. This format allows quick and efficient capture of distinct WBC subpopulations from small samples (similar to 30 mu L) of whole blood in a geometry that does not require any specialized equipment such as cell sorters or microfluidic devices.

引用

页码：17178 / 17183

页数：6

共 31 条

[1]

Neutrophil migration assay from a drop of blood [J].