CLASP Interacts with Sorting Nexin 1 to Link Microtubules and Auxin Transport via PIN2 Recycling in Arabidopsis thaliana

被引：123

作者：

Ambrose, Chris ^{[1
]}

Ruan, Yuan ^{[1
]}

Gardiner, John ^{[2
]}

Tamblyn, Laura M. ^{[1
]}

Catching, Amanda ^{[1
]}

Kirik, Viktor ^{[3
]}

Marc, Jan ^{[2
]}

Overall, Robyn ^{[2
]}

Wasteneys, Geoffrey O. ^{[1
]}

机构：

[1] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada

[2] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia

[3] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA

来源：

DEVELOPMENTAL CELL | 2013年 / 24卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CELL-DIVISION; POLARITY; CARRIER; GOLGI; TRAFFICKING; ENDOSOME; PATHWAY; GROWTH;

D O I：

10.1016/j.devcel.2013.02.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polarized movement of auxin generates concentration gradients within plant tissues to control cell division patterns and growth direction by modulating microtubule organization. In this study, we identify a reverse mechanism, wherein microtubules influence polar auxin transport. We show that the microtubule-associated protein CLASP interacts with the retromer component sorting nexin 1 (SNX1) to mediate an association between endosomes and microtubules. clasp-1 null mutants display aberrant SNX1 endosomes, as do wild-type plants treated with microtubule-depolymerizing drugs. Consistent with SNX1's role in trafficking of the auxin efflux carrier PIN-FORMED2 (PIN2), clasp-1 mutant plants have enhanced PIN2 degradation, and PIN2 movement to lytic vacuoles is rapidly induced by depolymerization of microtubules. clasp-1 mutants display aberrant auxin distribution and exhibit numerous auxin-related phenotypes. In addition to mechanistically linking auxin transport and microtubules, our data identify a ubiquitous endosome-microtubule association in plants.

引用

页码：649 / 659

页数：11