High-performance liquid chromatographic analysis of retinal and retinol isomers

被引:45
作者
Noll, GN
机构
[1] Physiologisches Institut, Justus-Liebig-Universität, D-35392 Giessen
关键词
mobile phase composition; retinol; retinal; vitamin A;
D O I
10.1016/0021-9673(95)00789-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Normal-phase chromatograms of retinal- and retinol isomers using various mobile phases are presented, showing that in n-hexane-tert.-butyl methyl ether and also in n-heptane-tert.-butyl methyl ether the elution order for retinol is 13-cis-, 11-cis-, 9-cis- and all-trans-retinol, whereas in n-hexane-1,4-dioxane 11-cis- elutes before 13-cis-retinol. Assignment of these main retinal and retinol peaks was performed using pure crystals of isomers, measurement of absorbance spectra and analysis of NMR spectra. The new mobile phase n-heptane-tert.-butyl methyl ether allows best baseline separation of the commonly occurring isomeric forms in reasonably short analysis time. In addition, eight di-cis- and tri-cis-retinol isomers were tentatively identified. The chromatograms show that former identifications in the literature are inconsistent or wrong.
引用
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页码:247 / 259
页数:13
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