Age- and sex-specific promoter function of a 2-kilobase 5′-flanking sequence of the murine luteinizing hormone receptor gene in transgenic mice

被引:28
作者
Hämäläinen, T [1 ]
Poutanen, M [1 ]
Huhtaniemi, I [1 ]
机构
[1] Univ Turku, Dept Physiol, FIN-20520 Turku, Finland
关键词
D O I
10.1210/en.140.11.5322
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A transgenic (TG) mouse model carrying a 2-kb murine LH receptor (LHR) promoter/beta-galactosidase (beta-GAL) fusion gene was created to study the regulatory function of the 5'-flanking region of the murine LHR gene. Of the five TG mouse Lines produced, three displayed high beta-GAL expression in the testis, but none showed any expression in the ovary. In addition, all mouse lines of both sexes expressed beta-GAL consistently in the brain, most prominently in hippocampus, hypothalamus, midbrain, and cortex. Weak staining was found in a few pituitary samples. All other tissues examined were negative for transgene expression. In support of sex-specific gonadal expression of the transgene, transient transfection of the LHR/beta-GAL gene construct into immortalized mouse granulosa (KK-1) and Leydig (mLTC-1) tumor cells revealed a more than 5-fold higher expression level in the Leydig cells. Histological examination of the TG testes demonstrated strong beta-GAL expression in Leydig cells, but, unexpectedly, also in elongating spermatids of adult age and in some spermatogonia of the neonatal testis. The functional significance of the latter findings remains open. The transgene was only expressed in adult Leydig cells; no expression was found in the fetal population of these cells. Hence, these findings indicate that the immediate 2-kb fragment of the murine LHR 5'-flanking sequence is transcriptionally active only in adult Leydig cells and certain brain areas, but of her promoter sequences are apparently needed for ovarian and fetal testicular expression of the LHR gene.
引用
收藏
页码:5322 / 5329
页数:8
相关论文
共 47 条
[31]   ONTOGENY OF GONADOTROPIN RECEPTORS AND GONADOTROPIN-STIMULATED CYCLIC-AMP PRODUCTION IN THE NEONATAL RAT OVARY [J].
SOKKA, T ;
HUHTANIEMI, I .
JOURNAL OF ENDOCRINOLOGY, 1990, 127 (02) :297-303
[32]   Development of luteinizing hormone action in the perinatal rat ovary [J].
Sokka, TA ;
Hamalainen, TM ;
Kaipia, A ;
Warren, DW ;
Huhtaniemi, IT .
BIOLOGY OF REPRODUCTION, 1996, 55 (03) :663-670
[33]  
Tao YX, 1998, J ANDROL, V19, P343
[34]  
TEERDS KJ, 1989, J ANDROL, V10, P472
[35]   Molecular mechanisms of reappearance of luteinizing hormone receptor expression and function in rat testis after selective Leydig cell destruction by ethylene dimethane sulfonate [J].
TenaSempere, M ;
Rannikko, A ;
Kero, J ;
Zhang, FP ;
Huhtaniemi, IT .
ENDOCRINOLOGY, 1997, 138 (08) :3340-3348
[36]   PERSISTENT EXPRESSION OF A TRUNCATED FORM OF THE LUTEINIZING-HORMONE RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE RAT TESTIS AFTER SELECTIVE LEYDIG-CELL DESTRUCTION BY ETHYLENE DIMETHANE SULFONATE [J].
TENASEMPERE, M ;
ZHANG, FP ;
HUHTANIEMI, I .
ENDOCRINOLOGY, 1994, 135 (03) :1018-1024
[37]   EXPRESSION OF FUNCTIONAL HUMAN CHORIONIC-GONADOTROPIN HUMAN LUTEINIZING-HORMONE RECEPTOR GENE IN HUMAN UTERINE ARTERIES [J].
TOTH, P ;
LI, X ;
RAO, CV ;
LINCOLN, SR ;
SANFILIPPO, JS ;
SPINNATO, JA ;
YUSSMAN, MA .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1994, 79 (01) :307-315
[38]   A novel human luteinizing hormone receptor gene [J].
Tsai-Morris, CH ;
Geng, Y ;
Buczko, E ;
Dufau, ML .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (01) :288-291
[39]  
TSAIMORRIS CH, 1991, J BIOL CHEM, V266, P11355
[40]   COMPARISON OF TOTAL SEQUENCE OF A CLONED RABBIT BETA-GLOBIN GENE AND ITS FLANKING REGIONS WITH A HOMOLOGOUS MOUSE SEQUENCE [J].
VANOOYEN, A ;
VANDENBERG, J ;
MANTEI, N ;
WEISSMANN, C .
SCIENCE, 1979, 206 (4416) :337-344