Human erythrocyte glycosphingolipids as alternative cofactors for human immunodeficiency virus type 1 (HIV-1) entry:: Evidence for CD4-Induced interactions between HIV-1 gp120 and reconstituted membrane microdomains of glycosphingolipids (gb3 and GM3)

被引：108

作者：

Hammache, D

Yahi, N

Maresca, M

Piéroni, G

Fantini, J ^{[1
]}

机构：

[1] Fac Sci St Jerome, Lab Biochim & Biol Nutr, ESA CNRS 6033, F-13397 Marseille 20, France

[2] Hop Enfants La Timone, UF SIDA, Virol Lab, F-13005 Marseille, France

[3] INSERM U130, F-13009 Marseille, France

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 06期

关键词：

D O I：

10.1128/JVI.73.6.5244-5248.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Glycosphingolipids from human erythrocytes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins. To identify the glycosphingolipid(s) which participates in the fusion process, we have analyzed the interaction of HIV-1 gp120 (X4 and R5X4 isolates) with reconstituted membrane microdomains of human erythrocyte glycosphingolipids. We identified globotriaosylceramide (Gb3) and ganglioside GM3 as the main glycosphingolipids recognized by gp120. In the presence of CD4, Gb3 interacted preferentially with the X4 gp120, whereas GM3 interacted exclusively with the R5X4 gp120. These data suggest that glycosphingolipid microdomains are required in CD4-dependent fusion and that Gb3 and/or GM3 may function as alternative entry cofactors for selected HIV-1 isolates.

引用

页码：5244 / 5248

页数：5

共 30 条

[11] HIV-1 Entry Cofactor: Functional cDNA Cloing of a Seven-Transmembrane, G protein-Coupled Receptor [J].