Bombesin peptide antagonist for target-selective delivery of liposomal doxorubicin on cancer cells

被引：33

作者：

Accardo, Antonella ^{[1
,2
,3
]}

Mansi, Rosalba ^{[4
]}

Salzano, Giuseppina ^{[5
]}

Morisco, Anna ^{[6
]}

Aurilio, Michela ^{[6
]}

Parisi, Antonio ^{[7
]}

Maione, Francesco ^{[7
]}

Cicala, Carla ^{[7
]}

Ziaco, Barbara ^{[3
]}

Tesauro, Diego ^{[1
,2
,3
]}

Aloj, Luigi ^{[6
]}

De Rosa, Giuseppe ^{[5
]}

Morelli, Giancarlo ^{[1
,2
,3
]}

机构：

[1] Univ Naples Federico II, Dept Biol Sci, CIRPeB, Naples, Italy

[2] Univ Naples Federico II, CNR, IBB, Naples, Italy

[3] Invectors Srl, Naples, Italy

[4] Univ Basel Hosp, Div Radiol Chem, CH-4031 Basel, Switzerland

[5] Univ Naples Federico II, Dept Pharmaceut & Toxicol Chem, Naples, Italy

[6] Fdn G Pascale, Ist Nazl Studio & Cura Tumori, Dept Nucl Med, Naples, Italy

[7] Univ Naples Federico II, Dept Expt Pharmacol, Naples, Italy

来源：

JOURNAL OF DRUG TARGETING | 2013年 / 21卷 / 03期

关键词：

Liposomes for drug delivery; bombesin peptide; doxorubicin delivery; anticancer efficacy; animal studies; GASTRIN-RELEASING-PEPTIDE; GOLD NANOPARTICLES; RECEPTOR ANTAGONISTS; POSITIVE TUMORS; HUMAN PROSTATE; IN-VITRO; CLONING;

D O I：

10.3109/1061186X.2012.741138

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purpose: This study addresses novel peptide modified liposomal doxorubicin to specifically target tissues overexpressing bombesin (BN) receptors. Methods: DOTA-(AEEA)(2)-peptides containing the [7-14]bombesin and the new BN-AA1 sequence have been synthesized to compare their binding properties and in serum stabilities. The amphiphilic peptide derivative (MonY-BN-AA1) containing BN-AA1, a hydrophobic moiety, polyethylenglycole (PEG), and diethylenetriaminepentaacetate (DTPA), has been synthesized. Liposomes have been obtained by mixing of MonY-BN-AA1 with 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Results: Both In-111 labeled peptide derivatives present nanomolar Kd to PC-3 cells. Lu-177 labeled peptide DOTA-(AEEA)(2)-BN-AA1 is very stable (half-life 414.1 h), while DOTA-(AEEA)(2)-BN, shows a half-life of 15.5 h. In vivo studies on the therapeutic efficacy of DSPC/MonY-BN-AA1/Dox in comparison to DSPC/MonY-BN/Dox, were performed in PC-3 xenograft bearing mice. Both formulations showed similar tumor growth inhibition (TGI) compared to control animals treated with non-targeted DSPC/Dox liposomes or saline solution. For DSPC/MonY-BN-AA1/Dox the maximum effect was observed 19 days after treatment. Conclusions: DSPC/MonY-BN-AA1/Dox nanovectors confirm the ability to selectively target and provide therapeutic efficacy in mice. The lack of receptor activation and possible acute biological side effects provided by using the AA1 antagonist bombesin sequence should provide safe working conditions for further development of this class of drug delivery vehicles.

引用

页码：240 / 249

页数：10

共 28 条

[21] CLONING OF A RECEPTOR FOR AMPHIBIAN [PHE(13)]BOMBESIN DISTINCT FROM THE RECEPTOR FOR GASTRIN-RELEASING PEPTIDE - IDENTIFICATION OF A 4TH BOMBESIN RECEPTOR SUBTYPE (BB4) [J].