Extraction of Puerarin using Ionic Liquid Based Aqueous Two-Phase Systems

被引：13

作者：

Fan, Jie-Ping ^{[1
,2
]}

Cao, Jing ^{[2
]}

Zhang, Xue-Hong ^{[3
]}

Huang, Jun-Zhong ^{[4
]}

Kong, Tao ^{[2
]}

Tong, Sheng ^{[2
]}

Tian, Zhe-You ^{[2
]}

Zhu, Jian-Hang ^{[1
,2
]}

Ouyang, Xiao-Kun ^{[5
]}

机构：

[1] Nanchang Univ, Key Lab Poyang Lake Ecol & Bioresource Utilizat, Minist Educ, Nanchang, Peoples R China

[2] Nanchang Univ, Sch Environm & Chem Engn, Nanchang, Peoples R China

[3] Nanchang Univ, Sch Foreign Language, Nanchang, Peoples R China

[4] Guang Dong Inst Drug Control, Guangzhou, Guangdong, Peoples R China

[5] Zhejiang Ocean Univ, Sch Food & Pharm, Zhoushan, Peoples R China

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2012年 / 47卷 / 12期

基金：

中国国家自然科学基金;

关键词：

aqueous two-phase systems; extraction; ionic liquids; puerarin; BIPHASIC SYSTEMS; BEHAVIOR; SEPARATIONS; CITRATE; SODIUM; WATER;

D O I：

10.1080/01496395.2012.659787

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Various ionic liquid-based aqueous two-phase systems (IL-ATPS) were evaluated to extract puerarin. The results indicated that the nature of ILs, the salting-out ability of salt, and the acidity and basicity of the salt-rich solution had important influence on the extraction efficiency. Various factors were optimized systematically, that is, the amount of IL, salt, puerarin, and short-chain alcohol. Under the optimal experimental conditions (the amount of K2HPO4 0.30-0.42 g/mL, [Bmim]Br 0.30-0.36 g/mL and 1.0 mL puerarin stock solution), the extraction efficiency of puerarin was over 99% by a single-step extraction, which indicated that the evaluated IL-ATPS was a prospective extraction medium. Finally, the IL-ATPS was successfully used to extract puerarin from Radix Puerariae Lobatae extracts.

引用

页码：1740 / 1747

页数：8

共 31 条

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Recovery in aqueous two-phase systems of lutein produced by the green microalga Chlorella protothecoides [J].