Sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of vinorelbine plasma concentrations

被引：23

作者：

Robieux, I ^{[1
]}

Vitali, V ^{[1
]}

Aita, P ^{[1
]}

Freschi, A ^{[1
]}

Lazzarini, R ^{[1
]}

Sorio, R ^{[1
]}

机构：

[1] CTR RIFERIMENTO ONCOL,IST TUMORI CTR EUROPEO,I-33081 AVIANO,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 675卷 / 01期

关键词：

vinorelbine;

D O I：

10.1016/0378-4347(95)00332-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method with fluorescence detection for the determination of vinorelbine in plasma is described. The technique was derived from that published by Debal for an assay of vinorelbine in cell culture medium. The modifications concern the preparation procedure for plasma samples (a two-step liquid-liquid extraction from plasma is described), optimization of the mobile phase composition, and use of a single C-18 column. These changes resulted in an improved sensitivity and reproducibility of the assay and led to its feasibility for clinical pharmacokinetic studies. The range of the assay is 2 to 1000 ng/ml.

引用

页码：183 / 187

页数：5

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