Plasmodium falciparum and Hyaloperonospora parasitica effector translocation motifs are functional in Phytophthora infestans

被引:60
作者
Grouffaud, Severine [1 ,2 ]
van West, Pieter [2 ]
Avrova, Anna O. [1 ]
Birch, Paul R. J. [3 ]
Whisson, Stephen C. [1 ]
机构
[1] Scottish Crop Res Inst, Plant Pathol Programme, Dundee DD2 5DA, Scotland
[2] Univ Aberdeen, Inst Med Sci, Aberdeen Oomycete Grp, Aberdeen AB25 2ZD, Scotland
[3] Univ Dundee, Scottish Crop Res Inst, Div Plant Sci, Dundee DD2 5DA, Scotland
来源
MICROBIOLOGY-SGM | 2008年 / 154卷
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1099/mic.0.2008/021964-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The oomycete potato late blight pathogen, Phytophthora infestans, and the apicomplexan malaria parasite Plasmodium falciparum translocate effector proteins inside host cells, presumably to the benefit of the pathogen or parasite. Many oomycete candidate secreted effector proteins possess a peptide domain with the core conserved motif, RxLR, located near the N-terminal secretion signal peptide. In the Ph. infestans effector Avr3a, RxLR and an additional EER motif are essential for translocation into host cells during infection. Avr3a is recognized in the host cytoplasm by the R3a resistance protein. We have exploited this cytoplasmic recognition to report on replacement of the RxLR-EER of Avr3a with the equivalent sequences from the intracellular effectors ATR1NdWsB and ATR13 from the related oomycete pathogen, Hyaloperotiospora parasitica, and the host targeting signal from the Pl. falciparum virulence protein PfHRPII. Introduction of these chimeric transgenes into Ph. infestans and subsequent virulence testing on potato plants expressing R3a demonstrated the alternative motifs to be functional in translocating Avr3a inside plant cells. These results suggest common mechanisms for protein translocation in both malaria and oomycete pathosystems.
引用
收藏
页码:3743 / 3751
页数:9
相关论文
共 42 条
[41]   A translocation signal for delivery of oomycete effector proteins into host plant cells [J].
Whisson, Stephen C. ;
Boevink, Petra C. ;
Moleleki, Lucy ;
Avrova, Anna O. ;
Morales, Juan G. ;
Gilroy, Eleanor M. ;
Armstrong, Miles R. ;
Grouffaud, Severine ;
van West, Pieter ;
Chapman, Sean ;
Hein, Ingo ;
Toth, Ian K. ;
Pritchard, Leighton ;
Birch, Paul R. J. .
NATURE, 2007, 450 (7166) :115-+
[42]   Adaptive evolution has targeted the C-terminal domain of the RXLR effectors of plant pathogenic oomycetes [J].
Win, Joe ;
Morgan, William ;
Bos, Jorunn ;
Krasileva, Ksenia V. ;
Cano, Liliana M. ;
Chaparro-Garcia, Angela ;
Ammar, Randa ;
Staskawicz, Brian J. ;
Kamoun, Sophien .
PLANT CELL, 2007, 19 (08) :2349-2369