Click oligo(ethylene glycol): An excellent orthogonal stationary phase to C18 for two-dimensional reversed-phase/reversed-phase liquid chromatography

被引：42

作者：

Liu, Yanming ^{[1
]}

Guo, Zhimou ^{[1
]}

Jin, Yu ^{[1
]}

Xue, Xingya ^{[1
]}

Xu, Qing ^{[1
]}

Zhang, Feifang ^{[1
]}

Liang, Xinmiao ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1206卷 / 02期

关键词：

Click OEG stationary phase; oligo(ethylene glycol); orthogonality; RP/RP; two-dimensional liquid chromatography; traditional Chinese medicine;

D O I：

10.1016/j.chroma.2008.08.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel click oligo(ethylene glycol) (Click OEG) stationary phase was proposed for constructing two-dimensional reversed-phase/reversed-phase liquid chromatography system (2D-RP/RPLC) combined with C18. The new stationary phase was prepared by immobilization of oligo(ethylene glycol) on silica support via click chemistry. And the resulting material was characterized by FT-IR, solid state CP/MAS C-13 NMR and elemental analysis. The investigation on orthogonality between Click OEG and C18 was performed by geometric approach with a set of testsolutes. The orthogonality reached 68.94%, which is much higher than other combinations. An offline 2DLC system consisted of Click OEG and C18 was constructed and applied to the analysis of a kind of traditional Chinese medicine Lignum Dalbergiae Odoriferae. Excellent separation results validated the high orthogonality between the Click OEG and C18 in the practical separation of complex samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：153 / 159

页数：7

共 47 条

[1] AUTOMATED INSTRUMENTATION FOR COMPREHENSIVE 2-DIMENSIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS [J].

BUSHEY, MM ;

JORGENSON, JW .

ANALYTICAL CHEMISTRY, 1990, 62 (02) :161-167

[2] Comprehensive two-dimensional liquid chromatography with parallel gradients for separation of phenolic and flavone antioxidants [J].

Cacciola, F. ;

Jandera, P. ;

Hajdu, Z. ;

Cesla, P. ;

Mondello, L. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1149 (01) :73-87

[3] Development of different comprehensive two dimensional systems for the separation of phenolic antioxidants [J].

Cacciola, Francesco ;

Jandera, Pavel ;

Blahova, Eva ;

Mondello, Luigi .

JOURNAL OF SEPARATION SCIENCE, 2006, 29 (16) :2500-2513

[4] Comprehensive multi-dimensional liquid chromatographic separation in biomedical and pharmaceutical analysis: a review [J].

Dixon, Steven P. ;

Pitfield, Ian D. ;

Perrett, David .

BIOMEDICAL CHROMATOGRAPHY, 2006, 20 (6-7) :508-529

[5] Comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in the analyses of triacylglycerols in natural lipidic matrixes [J].

Dugo, P ;

Kumm, T ;

Crupi, ML ;

Cotroneo, A ;

Mondello, L .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1112 (1-2) :269-275

[6] 2-DIMENSIONAL COLUMN LIQUID-CHROMATOGRAPHIC TECHNIQUE FOR RESOLUTION OF COMPLEX-MIXTURES [J].

ERNI, F ;

FREI, RW .

JOURNAL OF CHROMATOGRAPHY, 1978, 149 (FEB) :561-569

[7] SAMPLE DIMENSIONALITY - A PREDICTOR OF ORDER-DISORDER IN COMPONENT PEAK DISTRIBUTION IN MULTIDIMENSIONAL SEPARATION [J].

GIDDINGS, JC .

JOURNAL OF CHROMATOGRAPHY A, 1995, 703 (1-2) :3-15

[8] Orthogonality of separation in two-dimensional liquid chromatography [J].

Gilar, M ;

Olivova, P ;

Daly, AE ;

Gebler, JC .

ANALYTICAL CHEMISTRY, 2005, 77 (19) :6426-6434

[9] Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimensions [J].

Gilar, M ;

Olivova, P ;

Daly, AE ;

Gebler, JC .

JOURNAL OF SEPARATION SCIENCE, 2005, 28 (14) :1694-1703

[10] Two dimensional reversed-phase-reversed-phase separations -: Isomeric separations incorporating C18 and carbon clad zirconia stationary phases [J].

Gray, M ;

Dennis, GR ;

Wormell, P ;

Shalliker, RA ;

Slonecker, P .

JOURNAL OF CHROMATOGRAPHY A, 2002, 975 (02) :285-297

← 1 2 3 4 5 →