Regulation of early complement components C3 and C4 in the synovium

被引：13

作者：

Collins, T

Winkelstein, JA

Sullivan, KE

机构：

[1] CHILDRENS HOSP, DEPT PEDIAT, DIV ALLERGY IMMUNOL & INFECT DIS, PHILADELPHIA, PA 19104 USA

[2] JOHNS HOPKINS UNIV, SCH MED, DEPT PEDIAT, DIV ALLERGY & IMMUNOL, BALTIMORE, MD 21205 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1996年 / 3卷 / 01期

关键词：

D O I：

10.1128/CDLI.3.1.5-9.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To determine the cytokine inducibility of early complement component (C3 and C4) expression in the synovium, explant tissue was maintained in culture for 7 days. C3 and C4 production was measured by specific enzyme-linked immunosorbent assay, and RNA was evaluated by semiquantitative PCR, The effects of leukemia inhibitory factor (LIF), gamma interferon (IFN-gamma), IFN-alpha, and estrogen on C3 and C4 expression were evaluated. C3 levels were unaffected by 7 days of LIF, IFN-gamma, or IFN-alpha treatment. In contrast, C4 levels were significantly induced in synovial samples treated for 7 days with either IFN-gamma or IFN-alpha. LIF had no effect on C4 levels in this system. Estrogen was found to down-modulate the induction of expression due to IFN-gamma. These data provide evidence for cytokine regulation of C4 expression in the synovium and for estrogen modulation of those effects.

引用

页码：5 / 9

页数：5

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