Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast

被引：266

作者：

Roguev, Assen ^{[8
,9
]}

Bandyopadhyay, Sourav ^{[1
,2
]}

Zofall, Martin ^{[3
]}

Zhang, Ke ^{[3
]}

Fischer, Tamas ^{[3
]}

Collins, Sean R. ^{[4
,8
,9
]}

Qu, Hongjing ^{[8
,9
]}

Shales, Michael ^{[8
,9
]}

Park, Han-Oh ^{[5
]}

Hayles, Jacqueline ^{[6
]}

Hoe, Kwang-Lae ^{[7
]}

Kim, Dong-Uk ^{[7
]}

Ideker, Trey ^{[1
,2
]}

Grewal, Shiv I. ^{[3
]}

Weissman, Jonathan S. ^{[4
,8
,9
]}

Krogan, Nevan J. ^{[8
,9
]}

机构：

[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Program Bioinformat, La Jolla, CA 92093 USA

[3] NCI, Biochem & Mol Biol Lab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA

[4] Howard Hughes Med Inst, San Francisco, CA 94158 USA

[5] Bioneer Corp, Taejon, South Korea

[6] London Res Inst, Canc Res UK, Cell Cycle Lab, London WC2A 3PX, England

[7] Korea Res Inst Biosci & Biotechnol, Genom Res Ctr, Taejon, South Korea

[8] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94158 USA

[9] Univ Calif San Francisco, Calif Inst Quantitat Biosci, San Francisco, CA 94158 USA

来源：

SCIENCE | 2008年 / 322卷 / 5900期

关键词：

D O I：

10.1126/science.1162609

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.

引用

页码：405 / 410

页数：6

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