Achieving rapid low-pressure ion chromatography separations on short silica-based monolithic columns

被引：39

作者：

Pelletier, Sarah ^{[1
]}

Lucy, Charles A. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Gunning Lemieux Chem Ctr, Edmonton, AB T6G 2G2, Canada

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1118卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ion chromatography; short monolithic columns; fast separations; anions; extra-column band broadening; coating stability; flow injection analysis (FIA);

D O I：

10.1016/j.chroma.2006.03.072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Short silica-based monolithic columns (0.5-1 cm) are coated with the surfactant didodecyldimethylammonium bromide (DDAB) and used for fast ion exchange separations of small inorganic anions. Sources of extra-column band broadening are assessed and minimized to obtain separations of seven analytes (iodate, chloride, nitrate, bromide, nitrite, phosphate, sulphate) in two minutes at 2 mL/min. Eluents used are 6 and 9 mM 4-cyanophenol at pH 7.3-7.4 or 5 mM 4-hydroxybenzoic acid at pH 5.6. DDAB coating stability is improved by 15-fold by the addition of a DDAB coated pre-column before the injection valve. Separations are obtained using a low-pressure glass syringe. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：12 / 18

页数：7

共 42 条

[1] COLUMN EFFICIENCY MEASUREMENT [J].

BIDLINGMEYER, BA ;

WARREN, FV .

ANALYTICAL CHEMISTRY, 1984, 56 (14) :A583-&

[2] Applications of silica-based monolithic HPLC columns [J].

Cabrera, K .

JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :843-852

[3] DYNAMIC AND FIXED-SITE ION-EXCHANGE COLUMNS WITH CONDUCTOMETRIC DETECTION FOR THE SEPARATION OF INORGANIC ANIONS [J].

CASSIDY, RM ;

ELCHUK, S .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1983, 21 (10) :454-459

[4]

Conder J.R.C.L. Young., 1979, PHYSICOCHEMICAL MEAS

[5] Rapid, low pressure, and simultaneous ion chromatography of common inorganic anions and cations on short permanently coated monolithic columns [J].

Connolly, D ;

Victory, D ;

Paull, B .

JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :912-920

[6] Fast ion chromatography of common inorganic anions on a short ODS column permanently coated with didodecyldimethylammonium bromide [J].

Connolly, D ;

Paull, B .

JOURNAL OF CHROMATOGRAPHY A, 2002, 953 (1-2) :299-303

[7]

Dolan JW, 2004, LC GC N AM, V22, P26

[8] EQUATIONS FOR CALCULATION OF CHROMATOGRAPHIC FIGURES OF MERIT FOR IDEAL AND SKEWED PEAKS [J].

FOLEY, JP ;

DORSEY, JG .

ANALYTICAL CHEMISTRY, 1983, 55 (04) :730-737

[9]

GRANT CL, 1991, AM LAB, V23, P15

[10] Study of the mass transfer kinetics in a monolithic column [J].

Gritti, F ;

Piatkowski, W ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 2003, 983 (1-2) :51-71

← 1 2 3 4 5 →